IFN-γ Mouse anti-Human, APC, Clone: 4S.B3, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 551385

Catalog No. BDB551385

Description

Clone 4S.B3 reacts with the human form of interferon-γ (IFN-γ) detectable in the cytoplasm of a major subset of activated peripheral blood T lymphocytes. Clone 4S.B3 also cross-reacts with a cytoplasmic component of peripheral blood CD3+ lymphocytes of baboon, and both rhesus and cynomolgus macaque monkeys following five-hour treatment with phorbol myristic acetate (PMA) and Ca⁺;+ ionophore (A23187) in the presence of monensin. The staining pattern of 4S.B3 in CD3+ cells is similar to that observed with peripheral blood T lymphocytes from normal human donors. This reagent is useful for intracellular immunofluorescent staining for flow cytometric analysis to identify and enumerate IFN-γ+ cells within a mixed cell population. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Host Species: Mouse
Clone: 4S.B3
Isotype: IgG1 κ
Species Reactivity: Non-human primate
Immunogen: Partially purified human IFN-γ from supernatants of human PBMC stimulated with Staphylococcus aureus

Intracellular Staining

Specifications

• Antigen: IFN-γ
• Classification: Monoclonal
• Conjugate: APC
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: 4S.B3
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Immunogen: Partially purified human IFN-γ from supernatants of human PBMC stimulated with Staphylococcus aureus
• Quantity: 50 Tests
• Research Discipline: Immunology
• Target Species: Baboon, Cynomolgus, Rhesus Monkey
• Isotype: IgG1 κ

For Research Use Only.