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anti-IgG2a,κ, Clone: R35-95, BD

Manufacturer:  BD Biosciences 562952

Catalog No. BDB562952


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Description

Description

The R35-95 hybridoma was generated by hybridization of Y3 myeloma cells with spleen cells from LOU rats immunized with mouse immunoglobulins. The R35-95 hybridoma produces rat IgG2a,κ immunoglobulin that has no measurable reactivity with mouse immunoglobulins. The R35-95 immunoglobulin was selected as an isotype control following screening for low background binding on a variety of mouse and human tissues. The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 405nm and Em Max at 510nm, BD Horizon™ BV510 can be excited by the violet laser and detected in the BD Horizon™ V500 (525/50nm) filter set. BD Horizon™ BV510 conjugates are useful for the detection of dim markers off the violet laser.

Host Species: Rat
Clone: R35-95
Isotype: IgG2a, κ
Immunogen: Mouse Pooled Immunoglobulin

Flow Cytometry, Isotype Control

Specifications

Specifications

Control, Flow Cytometry
0.2mg/mL
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Rat
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype Control
R35-95
Brilliant Violet 510
Affinity Purified
Mouse Pooled Immunoglobulin
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV510 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV510 were removed.
RUO
Isotype Control
Monoclonal
SDS
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