Ki-67 Mouse anti-Human, Unlabeled, Clone: 35, BD

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB610968	BD Biosciences 610968	50 μg
BDB610969	BD Biosciences 610969	150 μg

Description

Cellular proliferation is a complex multi-faceted process, central to biological events ranging from embryonic development to wound healing. Although proliferation is tightly controlled, dysregulation often results in tumorigenesis. The regulatory process involves the expression of many cell-cycle-associated proteins that are subject to cycle-dependent modification. Such protein expression may be monitored to assess the proliferative capacity of cells, especially tumorigenic cells.The Ki-67 antigen is a nuclear protein expressed exclusively in proliferating cells during all active parts of the cell cycle. However, it is absent in quiescent cells and during DNA repair. The distribution of Ki-67 changes during different stages of the cell cycle. During G1, it is localized to the perinuclear region, but is primarily found in the nuclear matrix in later phases. During mitosis, it associates with the condensed chromosomes. The nuclear localization of Ki-67 and strict association with the cell cycle indicate its importance in the regulation of cell division. Therefore, Ki-67 has become an important marker of proliferating cells, and may also be a marker for disitinct nuclear matrix compartments.

Immunofluorescence, Western Blotting

Specifications

• Antigen: Ki-67
• Concentration: 250μg/mL
• Classification: Monoclonal
• Host Species: Mouse
• Research Discipline: Cell Biology
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Isotype: IgG1
• Purification Method: Affinity Purified

• Clone: 35
• Applications: Western Blot
• Conjugate: Unconjugated
• Regulatory Status: RUO
• Target Species: Human
• Immunogen: Human Ki-67 aa. 1547-1742
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

For Research Use Only.