anti-Ki-67, Clone: B56, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 556003

Catalog No. BDB556003

Description

The B56 monoclonal antibody reacts with the Ki-67 antigen, which is expressed in the nucleus of cycling cells. During G0 phase, the antigen cannot be detected. During interphase of the cell cycle, it is associated with nucleolar components, and it is on the surface of the chromosomes during M phase. Ki-67 is a large protein having 2 alternatively spliced isoforms, an N-terminal forkhead-associated domain, a C-terminal domain that binds to heterochromatin proteins, and multiple phosphorylation sites, the functions of which are still unclear. Because of the strict association of Ki-67 expression with cell proliferation, anti-Ki-67 antibodies are useful for the identification, quantification, and monitoring of growing cell populations. This antibody is routinely tested by flow cytometric and bioimaging analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Specifications

• Antigen: Ki-67
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Description: MKI67; Antigen identified by monoclonal antibody Ki-67; KIA
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

• Applications: In vitro Assay, Flow Cytometry
• Clone: B56
• Conjugate: Unconjugated
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Human Ki-67
• Quantity: 0.1 mg
• Research Discipline: Cell Biology
• Target Species: Human, Mouse, Rat, Rhesus Monkey
• Isotype: IgG1 κ