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Ly-6A/E Rat anti-Mouse, Unlabeled, Clone: D7, BD

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Description

Rat Monoclonal Antibody

The D7 antibody reacts with Ly-6A.2 and Ly-6E.1, which are allelic members of the Ly-6 multigene family. Sca-1 (Ly6A/E), a phosphatidylinositol-anchored protein of about 18kDa, is expressed on the multipotent hematopoietic stem cells (HSC) in the bone marrow of mice with both Ly-6 haplotypes. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, C57L, C58, DBA/2, PL, SJL, SWR, 129), Ly-6A/E is also expressed on distinct subpopulations of bone marrow and peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains with the Ly-6.1 haplotype (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas activation of lymphocytes from mice of both Ly-6 haplotypes leads to strong expression of the Sca-1 antigen. Studies with the D7 antibody have demonstrated that Ly-6A/E may be involved in the regulation of B and T lymphocyte responses, and it appears to be required for T-cell receptor-mediated T-cell activation. Purified E13-161.7 mAb (anti-Ly-6A/E, Mfr. No.553333) can block binding of FITC-conjugated D7 antibody (Mfr. No.557405) to mouse splenocytes, but purified mAb D7 is unable to block binding of FITC-conjugated E13-161.7 antibody (Mfr. No.553335). Anti-Ly-6A/E (Sca-1) mAb may be used in combination with the Mouse Lineage Panel (Mfr. No.559971) to identify HSC. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Host Species: Rat
Clone: D7
Isotype: IgG2a, κ
Cross Reactivity: Mouse

Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blotting

TRUSTED_SUSTAINABILITY

Specifications

Antigen Ly-6A/E
Applications Flow Cytometry
Classification Monoclonal
Clone D7
Concentration 0.5mg/mL
Conjugate Unconjugated
Description Ly-6A/E; Lymphocyte antigen 6A-2/6E-1; Ly-6A.2/Ly-6E.1; Sca-1; TAP
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species Rat
Immunogen IL-2-dependent mouse T-cell line CTL-L
Purification Method Affinity Purified
Quantity 0.1 mg
Regulatory Status RUO
Research Discipline Immunology
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C
Isotype IgG2a κ
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For Research Use Only.

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