Mac-3 Rat anti-Mouse, Unlabeled, Clone: M3/84, BD
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 553322
The M3/84 antibody reacts with the 110kDa Mac-3 antigen expressed on mouse mononuclear phagocytes. Surface expression of the 92 to 110kDa glycoprotein antigen increases during differentiation of monocytes to activated peritoneal macrophages. The M3/84 mAb can detect CD107b antigen on tissue macrophages, thioglycollate-elicited peritoneal macrophages, and some myeloid cell lines, but not on lymphocytes or monocytes. In the bone marrow, very few cells display CD107b antigen on the surface, but a large proportion express cytoplasmic CD107b. The M3/84 antibody has also been reported to stain dendritic cells and endothelium in sections of thymus (both medulla and cortex), lymph nodes, spleen (white pulp), and gut-associated lymphoid tissue.
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|Mouse C57Bl/6 peritoneal exudate cells|
|Store undiluted at 4°C|
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