Invitrogen™ Parvalbumin Polyclonal Antibody

Catalog No.	Quantity
PA1933	100 μg

Catalog No. PA1933 Supplier Invitrogen™ Supplier No. PA1933

Description

Rabbit Polyclonal Antibody

PA1-933 detects parvalbumin protein in human and rat samples. PA1-933 has successfully been used in Western blot, ELISA, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects a 12 kDa protein representing parvalbumin from rat cerebellum. Parvalbumin protein is relatively small and, therefore, it is recommended that the electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane. The PA1-933 immunizing protein corresponds to purified parvalbumin from rat skeletal muscle. Reconstitute in 100 μL PBS to create a stock of 1 mg/mL.

Parvalbumin (PV) is a calcium binding protein expressed in specific muscle fibers and fast-firing neurons. PV consists of a single, unbranched chain of linked amino acids and belongs to a larger group of EF hand proteins. Studies have demonstrated that parvalbumin acts in the decay of calcium in the contraction/ relaxation cycle of fast twitch muscles. This data has shown a positive correlation between the rate of relaxation and the concentration of parvalbumin. Parvalbumin is also expressed in a specific population of GABAergic interneurones which are thought to play a role in maintaining the balance between excitation and inhibition in the cortex as well as the hippocampus. In amyotrophic lateral sclerosis (ALS) patents, parvalbumin immunoreactivity is specifically absent from neuron populations lost early in ALS.

Specifications

• Antigen: Parvalbumin
• Applications: ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: 0.1M sodium phosphate with 20mg/mL BSA and 0.1% sodium azide; pH 7.0
• Gene: Pvalb
• Gene Accession No.: P02625, P20472
• Gene Alias: D22S749; HGNC:9704; MGC116759; PALB1; Parv; parvalbumin; Parvalbumin (calcium binding protein); parvalbumin alpha; PV; Pva; PVALB
• Gene Symbols: Pvalb
• Host Species: Rabbit
• Immunogen: Purified parvalbumin from rat skeletal muscle.
• Purification Method: Antigen Affinity Chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 25269, 5816
• Target Species: Human, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG