MilliporeSigma™ anti-PhosphoDetect RhoA (pSer188), Polyclonal

Catalog No.	Quantity	Conjugate
PS1089100UL	100 μL	Unconjugated

Catalog No. PS1089100UL Supplier MilliporeSigma™ Supplier No. PS1089100UL

Description

Rabbit Polyclonal Antibody

Specifically detects PhosphoDetect™ RhoA (pSer¹⁸⁸) Clone: in Human samples, and it is validated for ELISA, Immuno Blotting

Rho (A, B, and C) proteins are members of the Ras superfamily of GTPases. They regulate a variety of cellular functions, including cell cycle progression, cytoskeletal rearrangement, and gene expression. Rho cycles between the active GTP-bound form and the inactive GDP-bound form. Conversion from the inactive to the active form and vice versa is controlled by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The three Rho proteins are highly homologous and contain the consensus sequences necessary for GDP/GTP binding and GTPase activity. RhoA is phosphorylated at Ser¹⁸⁸in vitro by PKA and PKG. This phosphorylation enhances RhoGDI binding and inhibits RhoA-mediated stress fiber formation, indicating that Ser¹⁸⁸ is an important site for negative regulation of RhoA activity.

Specifications

• Antigen: PhosphoDetect™ RhoA (pSer¹⁸⁸)
• Applications: ELISA, Immunoblot, Immunoblot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: In PBS, 1mg/mL BSA, 50% glycerol.
• Host Species: Rabbit
• Immunogen: a synthetic peptide corresponding to amino acids surrounding the Ser¹⁸⁸ phosphorylation site of human RhoA, coupled to KLH
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes the ∽22kDa RhoA protein, expressed as a GST fusion protein (total ∽49kDa) and phosphorylatedin vitro by PKA.
• Target Species: Human
• Content And Storage: −20°C
• Form: Purified
• Isotype: IgG

For Research Use Only