PKA (RIIα) Mouse, Unlabeled, Clone: 40, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 612242

Catalog No. BDB612242

Description

cAMP-dependent Protein Kinase (PKA) is composed of two distinct subunits: catalytic(C) and regulatory (R). Four regulatory subunits have been identified: RIα, RIβ, RIIα, and RIIβ.These subunits define type I and II cAMP-dependent protein kinases. Following binding of cAMP, the regulatory subunits dissociate from the catalytic subunits, rendering the enzyme active. Type I and type II holoenzymes have three potential C subunits (Cα, Cβ, or Cγ). Type II PKA can be distinguished by autophosphorylation of the R-subunits, while type I PKA binds Mg/ATP with high affinity. Most cells express both type I and type II PKAs. Although the Rα isoforms are ubiquitously expressed, the Rβ isoforms are predominant in nervous and adipose tissues. Expression of the RIα subunit is modulated during muscle and adipocyte differentiation in vitro.

Immunofluorescence, Immunoprecipitation, Western Blotting

Specifications

• Antigen: PKA (RIIα)
• Classification: Monoclonal
• Concentration: 250μg/mL
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Immunogen: Human PKA[RIIα] aa. 1-404
• Quantity: 50 μg
• Research Discipline: Cell Biology
• Target Species: Canine, Human, Mouse, Rat
• Isotype: IgG1

• Applications: Western Blot
• Clone: 40
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.