PSMD3 Rabbit anti-Human, Mouse, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1974
PA1-974 detects proteasome 19S subunit S3 from human and mouse cells. PA1-974 has been successfully used in ICC/IF, FACS, and Western blot procedures. By Western blot, this antibody detects an ~60 kDa protein representing proteasome 19S subunit S3 from HeLa cell extract. For Western blot procedures it is necessary to transfer the protein to membrane at pH 11. PA1-974 immunizing peptide corresponds to amino acid residues 513-534 from human proteasome 19S subunit S3 protein.Proteolytic degradation is critical to the maintece of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman’s syndrome, and Liddle syndrome.
|Synthetic Peptide: E(513) R E Q Q D L E F A K E M A E D D D D S F P(534)|
|Antigen affinity chromatography|
|Flow Cytometry, Immunocytochemistry, Immunofluorescence, Western Blot|
|PBS with 1mg/mL BSA and 0.05% sodium azide|
|P58, RPN3, S3|
|-20° C, Avoid Freeze/Thaw Cycles|
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