Stat3 (pY705) Mouse, Alexa Fluor 488, Clone: 4/P-STAT3, BD

Catalog No.	Quantity
BDB557814	50 Tests

Catalog No. BDB557814 Supplier BD Biosciences Supplier No. 557814

Description

Mouse Monoclonal Antibody

Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat3 is a 92kDa protein that is activated as a DNA- binding protein through cytokines, such as IL-6, and growth factors, such as EGF. Stat3 activation occurs via tyrosine phosphorylation at Y705. Tyrosine phosphorylation in response to cytokine stimulation is generally mediated by JAK1. Upon activation, Stat3 dimerizes, translocates to the nucleus and binds DNA response elements, thereby regulating gene expression. It has been reported that Stat3 binds to DNA as a homodimer, but it is also capable of binding as a heterodimer with Stat1. In addition to tyrosine phosphorylation, Stat3 is also phosphorylated at S727 via the MAPK pathway. Stat3 is widely expressed and can bind to the sis-inducible element (SIE) site from the c-fos promoter. This site is similar to the GAS element that is present in IFN-γ induced genes. Thus, phosphorylation of Y705 in Stat3 occurs in response to growth factors and cytokines, and is essential for normal transcription activity. The 4/P-STAT3 monoclonal antibody recognizes the phosphorylated Y705 of Stat3. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Host Species: Mouse
Clone: 4/P-STAT3
Isotype: IgG2a, κ
Cross Reactivity: Human, Mouse

Flow Cytometry, Intracellular Staining, Western Blotting

Specifications

• Antigen: Stat3 (pY705)
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: 4/P-STAT3
• Conjugate: Alexa Fluor 488
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Host Species: Mouse
• Immunogen: Phosphorylated Human Stat3 Peptide
• Purification Method: Affinity Purified
• Quantity: 50 Tests
• Regulatory Status: RUO
• Research Discipline: Cell Biology
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2a κ

For Research Use Only.