CD8, CD4 and CD3 cocktail , APC, FITC, PE, Clone: SK1, BD

Monoclonal Antibody

Supplier:  BD Biosciences 558625

Catalog No. BDB558625

Description

Cocktail Component Clone Isotype

APC anti-Human CD8 SK1 mIgG1

FITC anti-Human CD3 SP34-2 mIgG1

The NHP T Lymphocyte Cocktail is a three-color reagent cocktail designed to identify NHP T lymphocytes by direct immunofluorescence staining with flow cytometric analysis. The SK1 antibody reacts with the hinge-like membrane-proximal domain of the 32-kDa alpha chain of the CD8 differentiation antigen.. The CD8α and β chains (CD8a and CD8b, respectively) form a heterodimer on the surface of most thymocytes and a subpopuplation of mature T-lymphocytes (ie, MHC class I-restricted T cells, including most T suppressor/cytotoxic cells). The L200 antibody reacts with the human form of the 56 kDa transmembrane glycoprotein, CD4, present on the T-helper/inducer subset of normal human donor peripheral blood lymphocytes. L200 antibody also cross-reacts with a subset of CD3-positive peripheral blood lymphocytes, but not monocytes, of both rhesus and cynomolgus macaque monkeys. Cross-reactivity on both lymphocytes and monocytes (weak) of baboon is also observed. The distribution on lymphocytes is similar for both human and monkey, with the majority of CD4-positive lymphocytes being CD8-negative and lacking reactivity with antibodies to B- or NK-cell markers. The SP34-2 antibody reacts with the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes and peripheral T lymphocytes. Clone SP34-2 is a mouse IgG1 isotype monoclonal antibody, descendant of SP34 (mouse IgG3), with the same specificity and reactivity pattern as the parent clone. It cross-reacts with a major subset of peripheral blood lymphocytes, but not monocytes or granulocytes, of baboon, and rhesus, cynomolgus, and pigtail macaque monkeys.

Specifications

• Antigen: CD8, CD4 and CD3 cocktail
• Classification: Monoclonal
• Conjugate: FITC/APC/PE
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Baboon, Cynomolgus, Rhesus Monkey
• Isotype: IgG1

• Applications: Flow Cytometry
• Clone: SK1
• Formulation: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
• Quantity: 50 Tests
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov