Invitrogen™ Anza™ DNA End Repair Kit

Catalog No. IVGN2504

Supplier: Invitrogen™ IVGN2504

Description

Used to convert DNA with cohesive ends to blunt-ended 5'-phosphorylated DNA for use in blunt-end ligation reactions

The Invitrogen™ Anza™ DNA End Repair Kit is used to convert DNA with cohesive ends to blunt-ended 5’-phosphorylated DNA for use in blunt-end ligation reactions. The included pre-mixed Anza™ DNA End Repair Mix contains T4 DNA polymerase, Klenow Fragment, and T4 PNK (polynucleotide kinase) to minimize pipetting, while the Anza™ 10X End Repair Buffer contains ATP and dNTPs to facilitate the activity of the enzyme mix.

Benefits:

• Convenient—pre-mixed enzyme mixture for superior convenience and reduction of pipetting
• Efficient—simultaneous blunting and phosphorylation
• Compatible—use directly in Anza restriction enzyme digestion reaction mixture following heat inactivation
• Fast—end repair complete in 15 minutes

The Anza DNA End Repair Kit is part of the Anza™ Restriction Enzyme Cloning System, unifying the traditional cloning processes. Other Anza DNA Modifying Enzymes include the Anza T4 DNA Ligase Master Mix, Anza T4 PNK Kit, and Anza DNA Blunt End Kit.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

• Content And Storage: 100 μL Anza DNA End Repair Mix
  200 μL Anza 10X End Repair Buffer
  
  Store at -5 to -30°C.
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Enzyme: T4 Polynucleotide Kinase, T4 DNA polymerase, Klenow Fragment
• Compatible Buffer: Anza™ 10X End Repair Buffer
• Quantity: 20 reactions
• Product Type: DNA End Repair Kit
• Product Line: Anza

For Research Use Only. Not for use in diagnostic procedures.