Apexbio Technology LLC Annexin V-APC/DAPI Apoptosis Kit 100tests

Supplier:  Apexbio Technology LLC K229420

Annexin V-APC/DAPI Apoptosis Kit allow discrimination between cells at various stages of apoptosis and cell death Annexin V is a calcium-dependent phospholipid-binding protein which specifically binds to phosphatidylserine (PS) Under normal conditions PS is located on the inner surface of the cell membrane lipid bilayer but during early apoptosis PS translocates from the inner leaflet to the outer leaflet of the plasma membrane becoming exposed to the extracellular environment By using Annexin V-APC labeling apoptotic cells can be detected easily through flow cytometry or fluorescence microscopy within 15-30 minutes Additionally this kit includes the nuclear stain DAPI which specifically binds to double-stranded DNA to emit blue fluorescence Normally DAPI cannot penetrate intact cell membranes In late apoptotic and dead cells DAPI can cross the compromised membrane to stain the nucleus

Catalog No. 50-310-7050