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Invitrogen™ APC Monoclonal Antibody (eBioAPC-6A2), eBioscience™, Invitrogen™
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Catalog No. 5013032
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50-130-32 50 μg
50-130-33 500 μg
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Catalog No. 50-130-32 Supplier Invitrogen™ Supplier No. 14417081
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Description

Mouse Monoclonal Antibody

Description: The eBioAPC-6A2 monoclonal antibody reacts with allophycocyanin (APC) and APC-Cy7, with no crossreactivity to fluorescein (FITC) or phycoerythrin (PE). When using eBioAPC-6A2 as a second-step antibody, in combination with an appropriate third step, to bind a APC-conjugated primary antibody, there is no decrease in the fluorescent intensity of the directly conjugated APC antibody. Therefore no interference or quenching of the APC fluorochrome is observed. The eBioAPC-6A2 can be used for separation of cells labeled with APC-conjugated antibodies or for staining. Please note that the optimal concentration for this antibody will vary depending on the density of the primary APC-conjugated antibody and the application tested. Applications Reported: This eBioAPC-6A2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioAPC-6A2 antibody has been tested flow cytometric analysis of normal human peripheral blood cells stained with an APC-conjugated antibody. This can be used at less than or equal to by μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE.

Anti-APC (Allophycocyanin) antibodies can be used to bind APC-conjugated primary antibodies without decreasing fluorescent intensity of the APC fluorochrome. This method is used for separation of cells labeled with APC-conjugated antibodies.
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Specifications

Antigen APC
Applications Flow Cytometry
Classification Monoclonal
Clone eBioAPC-6A2
Concentration 0.5 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene Alias APC
Host Species Mouse
Purification Method Affinity chromatography
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Tag
Content And Storage 4°C
Product Type Antibody
Form Liquid
Isotype IgG1
Target Molecule Tag
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