APC11, Mouse anti-Human, Clone: CRQ1.1, Millipore Sigma™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
MABE178925	MilliporeSigma MABE178925UG	25 μg
MABE1789100	MilliporeSigma MABE1789100UG	100 μg

Description

Anti-APC11, clone CRQ1.1, Cat. No. MABE1789, is a highly specific mouse monoclonal antibody that targets Anaphase-promoting complex subunit 11 and has been tested in Western Blotting.

Anaphase-promoting complex subunit 11 (UniProt: Q9NYG5; also known as APC11, Cyclosome subunit 11, Hepatocellular carcinoma-associated RING finger protein) is encoded by the ANAPC11 (also known as HSPC214) gene (Gene ID: 51529) in human. APC11 is one of the components of the anaphase-promoting complex (APC) that belongs to the RING-box family of proteins. APC is a large E3 ubiquitin ligase that responsible for targeting proteins for degradation during mitosis. The APC is assembled from 13 different proteins where in APC2 binds to APC11 forming the catalytic core that recruits the E2 ubiquitin conjugating enzyme. APC11 has with multiple zinc -binding sites with a RING-type zinc-finger domain (aa 34-77). It is expressed at high levels in the skeletal muscle and heart and in moderate levels in brain, kidney, and liver. Two isoforms of APC11 have been described that are produced by alternative splicing. (Ref.: Schreiber, A et al., (2011). Nature. 470(7333); 227-32).

Specifications

• Antigen: APC11
• Dilution: Western Blotting Analysis: A representative lot detected APC11 in Western Blotting applications (Sedgwick, G.G., et. al. (2013). EMBO J. 32(2):303-14).Western Blotting Analysis: A representative lot detected APC11 in APC11-Flag expressed in HeLa cells following tetracycline treatment.
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Alias: Anaphase-promoting complex subunit 11;Cyclosome subunit 11;Hepatocellular carcinoma-associated RING finger protein
• Gene Symbols: ANAPC11;HSPC214
• Isotype: IgG2a κ
• Purification Method: Protein G purified
• Test Specificity: Clone CRQ1.1 specifically detects human Anaphase-promoting complex subunit 11 (APC11). It targets an epitope with in 9 amino acids from the C-terminal region.

• Clone: CRQ1.1
• Applications: Western Blot
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Epigenetics & Nuclear Function
• Formulation: Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene ID (Entrez): NP_001002244.1
• Immunogen: A linear peptide corresponding to 9 amino acids from the C-terminal region of human Anaphase-promoting complex subunit 11 (APC11).
• Primary or Secondary: Primary
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Molecular Weight of Antigen: 9.84 kDa calculated.