Invitrogen™ ApoA1 Monoclonal Antibody (311)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MIA1402

Catalog No. PIMIA1402

Description

By sandwich ELISA, MIA1402 can be used as a detection antibody with Product # 710263 or # 701239 as a coating antibody, to generate a matched pair. Using these matched pairs, recombinant human Apo A-1, but not recombinant mouse Apo A-1, was detected. MIA1402 can be used to detect Apo A-1 from serum samples. To increase sensitivity of sandwich ELISAs with MIA1402, a biotinylated detection antibody followed by Streptavidin-HRP is recommended. By Western blot, MIA1402 detects recombinant human Apo A-1, but not recombinant mouse Apo A-1. MIA1402 may not be as successful for detecting endogenous Apo A-1 by Western blot. MIA1402 was formerly sold as a Seradyn product.

Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.

Specifications

• Antigen: ApoA1
• Classification: Monoclonal
• Concentration: 5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.4
• Gene Accession No.: P02647, Q00623
• Gene Symbols: APOA1
• Immunogen: Purified human plasma Apo A-1.
• Quantity: 1 mg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Radioimmune Assays (RIA), Western Blot, Immunocytochemistry
• Clone: 311
• Conjugate: Unconjugated
• Gene: APOA1
• Gene Alias: 1-Sep; 2-Sep; Alp-1; Apo a; APO A1; apo A-1; APO I; apo(a); APOA1; Apoa-1; ApoAI; Apo-AI; apoA-I; Apolipoprotein; Apolipoprotein A1; apolipoprotein A-1; Apolipoprotein AI; apolipoprotein A-I; apolipoprotein A-I precursor; Apolipoprotein A-I(1-242); Brp-14; Ltw-1; Lvtw-1; MGC102525; MGC117399; MGC124929; preproapolipoprotein A-I; prepro-apolipoprotein-AI; ProapoA-I; Proapolipoprotein A-I; Sep-1; Sep2; Sep-2; Truncated apolipoprotein A-I; Udpgt; Ugt1; Ugt1a7
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 11806, 335
• Content And Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Form: Liquid

Safety and Handling

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