Invitrogen™ ApoA1 Monoclonal Antibody (6001)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA514667

Catalog No. PIMA514667

Description

By sandwich ELISA, MA5-14667 can be used as a detection antibody with Product # 710263 as the coating antibody, to generate a matched pair. Using this matched pair, recombinant human Apo A-1, but not recombinant mouse Apo A-1, was detected. It is not recommended to use Product # 701239 for coating when MA5-14667 is used for detection. By sandwich ELISA, MA5-14667 can be used to detect Apo A-1 from serum samples. To increase sensitivity of sandwich ELISAs with MA5-14667, a biotinylated detection antibody followed by Streptavidin-HRP is recommended. By Western blot, MA5-14667 detects recombinant human Apo A-1, but not recombinant mouse Apo A-1. MA5-14667 may not be as successful for detecting endogenous Apo A-1 by Western blot. Product MA514667 is a smaller package size of MIA1401 (formerly sold as a Seradyn product).

Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.

Specifications

• Antigen: ApoA1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide; pH 7.4
• Gene Accession No.: P02647
• Gene Symbols: APOA1
• Immunogen: Purified human plasma Apo A-1.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a

• Applications: ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Radioimmune Assays (RIA), Western Blot
• Clone: 6001
• Conjugate: Unconjugated
• Gene: APOA1
• Gene Alias: 1-Sep; 2-Sep; Alp-1; Apo a; APO A1; apo A-1; APO I; apo(a); APOA1; Apoa-1; ApoAI; Apo-AI; apoA-I; Apolipoprotein; Apolipoprotein A1; apolipoprotein A-1; Apolipoprotein AI; apolipoprotein A-I; apolipoprotein A-I precursor; Apolipoprotein A-I(1-242); Brp-14; Ltw-1; Lvtw-1; MGC102525; MGC117399; MGC124929; preproapolipoprotein A-I; prepro-apolipoprotein-AI; ProapoA-I; Proapolipoprotein A-I; Sep-1; Sep2; Sep-2; Truncated apolipoprotein A-I; Udpgt; Ugt1; Ugt1a7
• Host Species: Mouse
• Purification Method: Ion-exchange chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 335
• Content And Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Form: Liquid