Applied Biosystems™ AmpliTaq™ 360 DNA Polymerase

Description

Includes

AmpliTaq 360 DNA Polymerase is supplied at 5U/μL with 10X AmpliTaq 360 PCR Buffer, 25mM M2Cl₂, and 360 GC Enhancer. Typically, 1.5mL of each of AmpliTaq 360 Buffer, 25mM MgCl₂, and 360 GC Enhancer is supplied with each 250U of enzyme. Storage Buffer Enzyme is supplied in 20mM Tris, pH 9.0, 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% (w/v) Tween 20, 50% (v/v) glycerol. Source: Recombinant, modified form of the Thermus aquaticus DNA polymerase gene expressed in E. coli. All solutions are prepared in deionized, filter-sterilized water.

The Applied Biosystems AmpliTaq360 DNA Polymerase, when used with the new enhanced AmpliTaq 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.

The Applied Biosystems AmpliTaq360 DNA Polymerase, when used with the new enhanced AmpliTaq 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. Compared to the original AmpliTaq DNA Polymerase, AmpliTaq 360 DNA Polymerase is purified by an additional proprietary separation process that reduces contaminating bacterial DNA sequences from the enzyme preparation. This ultra-pure enzyme reduces false positive results, amplifies low-level target sequences, and, when combined with the proprietary AmpliTaq 360 Buffer Kit, promotes the amplification of a variety of templates, including those from bacterial and human genomes.

Features

• Optimized for the broadest range of targets, from everyday to challenging
• Unmatched sensitivity and yield
• Market-leading GC enhancer for robust amplification of GC-rich sequences
• Achieves the highest quality sequencing data

Notes

• DreamTaq DNA Polymerase is recommended for superior PCR performance.

Order Info

Approved for shipment at Room Temperature or on Wet Ice

Specifications

• Concentration: 5 U/μL
• Content And Storage: • AmpliTaq 360 DNA Polymerase (5 U/μL), 50 μL
  • 10X AmpliTaq 360 PCR Buffer, 1.5 mL
  • 25 mM MgCl₂, 1.5 mL
  • 360 GC Enhancer, 1.5 mL
  
  Store at -15°C to -25°C.
  Product is guaranteed up to 12 months as indicated by the expiration date.
• Format: Stand-alone enzyme
• GC-Rich PCR Performance: Medium
• Polymerase: AmpliTaq 360 DNA Polymerase
• Reaction Speed: Standard
• Exonuclease Activity: 5' - 3'
• Green Features: Sustainable packaging
• Product Type: DNA Polymerase
• Quantity: 250 Units
• Shipping Condition: Room Temp or Wet Ice
• For Use With (Application): Standard PCR
• Fidelity (vs. Taq): 1X
• Hot Start: No
• No. of Reactions: 200 Reactions
• Overhang: 3'-A
• Reaction Format: Separate Components
• Size (Final Product): 5 kb or less
• Starting Material: DNA

For Research Use Only. Not for use in diagnostic procedures.