Applied Biosystems™ AmpliTaq™ DNA Polymerase with Buffer I or Buffer II

Catalog No.	Includes	Quantity
N8080172	Buffer II	1000 Units
N8080161	Buffer II	250 Units
N8080153	Buffer II	3,000 units
N8080156	Buffer II	5,000 units
N8080186	Buffer II	25,000 units
N8080152	Buffer I	3,000 units
N8080155	Buffer I	5,000 units
N8080160	Buffer I	250 Units
N8080171	Buffer I	1,000 units
N8080185	Buffer I	25,000 units

Catalog No. N8080172 Supplier Applied Biosystems™ Supplier No. N8080172

Description

Includes

Contains 1000 units of AmpliTaq DNA Polymerase, Buffer II, and MgCl₂ Solution.

AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli.

AmpliTaq DNA Polymerase is a 94-kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process, and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial and lot-to-lot.

AmpliTaq DNA Polymerase is available with GeneAmp10X PCR Buffer I or with GeneAmp 10X PCR Buffer II and MgCl₂ Solution.

Reliable and robust PCR

The thermal activity profile of AmpliTaq DNA Polymerase is good for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55–75°C). The enzyme's half-life is ∼40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications.

Features

• The most thoroughly characterized enzyme available for the PCR process
• Excellent thermal activity profile makes it good for PCR applications
• QC-tested to guarantee reproducible results

Notes

• For superior PCR performance, we recommend DreamTaq DNA Polymerase.

Order Info

Shipping Condition: Dry ice

Specifications

• Concentration: 5 U/μL
• Content And Storage: • AmpliTaq DNA Polymerase (5 U/μL), 200 μL
  • GeneAmp 10X PCR Buffer II, 4 x 1.5 mL
  • 25 mM MgCl₂, 4 x 1.5 mL
  
  Store at -20°C.
• Format: Stand-alone enzyme
• Polymerase: AmpliTaq DNA Polymerase
• Reaction Speed: Standard
• Exonuclease Activity: 5' - 3'
• Product Type: DNA Polymerase
• Quantity: 1000 Units
• Shipping Condition: Dry Ice
• For Use With (Application): Standard PCR
• Fidelity (vs. Taq): 1X
• Hot Start: No
• No. of Reactions: 800 Reactions
• Overhang: 3'-A
• Reaction Format: Separate Components
• Size (Final Product): 5 kb or less
• Starting Material: DNA
• Includes: Buffer II

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.