Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit

Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2μg total RNA

Catalog Number	Mfr. No.	No. of Reactions
43-688-14	Applied Biosystems™ 4368814	200 Reactions
43-688-13	Applied Biosystems™ 4368813	1,000 Reactions

Description

The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2μg of total RNA to single-stranded cDNA in single 20μL reaction. Reactions can be scaled up to 100μL to generate 10μg of cDNA from single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.

• Linear target amplification for real-time PCR
• Higher yields and precision than other cDNA synthesis kits at a fraction of the cost
• Ten-fold greater dynamic range than other kits
• Optimal reaction temperature: 37°C
• Final product size: 7kb or less

• Extensively tested with a variety of templates
• Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA

PCR & Real-Time PCR, Reverse Transcription

Order Info

Shipping Condition: Dry ice

Specifications

• Concentration: 50 U/μL
• Final Product Type: First-Strand cDNA
• PCR Method: RT-PCR, RT-qPCR
• Reaction Speed: 120 min.
• Starting Material: RNA
• Volume: 100 μL, 20 μL

• Detection Method: Primer-Probe
• Format: First-Strand cDNA Synthesis Kit
• Reaction Format: Separate components
• Reagent Type: Reverse Transcription
• Technique: Reverse Transcription
• Ribonuclease H Activity: Yes

For Research Use Only. Not for use in diagnostic procedures.