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Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit

Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2μg total RNA

$441.00 - $1696.00

Specifications

Concentration 50 U/μL
Detection Method Primer-Probe
Final Product Type First-Strand cDNA
Format First-Strand cDNA Synthesis Kit
PCR Method RT-PCR, RT-qPCR
View More Specs

Products 2
Catalog Number Mfr. No. No. of Reactions Price Quantity & Availability  
Catalog Number Mfr. No. No. of Reactions Price Quantity & Availability  
43-688-14
Encompass
View Documents
Applied Biosystems™
4368814
200 Reactions
Each for $441.00
Add to cart
 
43-688-13
Encompass
View Documents
Applied Biosystems™
4368813
1,000 Reactions
Each for $1,696.00
Add to cart
 
Description

Description

The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2μg of total RNA to single-stranded cDNA in single 20μL reaction. Reactions can be scaled up to 100μL to generate 10μg of cDNA from single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.

  • Linear target amplification for real-time PCR
  • Higher yields and precision than other cDNA synthesis kits at a fraction of the cost
  • Ten-fold greater dynamic range than other kits
  • Optimal reaction temperature: 37°C
  • Final product size: 7kb or less

  • Extensively tested with a variety of templates
  • Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA

PCR & Real-Time PCR, Reverse Transcription

Order Info

Shipping Condition: Dry ice

Specifications

Specifications

50 U/μL
First-Strand cDNA
RT-PCR, RT-qPCR
120 min.
RNA
100 μL, 20 μL
Primer-Probe
First-Strand cDNA Synthesis Kit
Separate components
Reverse Transcription
Reverse Transcription
Yes
SDS
Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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