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Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit
Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2μg total RNA
$441.00 - $1696.00
Specifications
Concentration | 50 U/μL |
---|---|
Detection Method | Primer-Probe |
Final Product Type | First-Strand cDNA |
Format | First-Strand cDNA Synthesis Kit |
PCR Method | RT-PCR, RT-qPCR |
Description
The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2μg of total RNA to single-stranded cDNA in single 20μL reaction. Reactions can be scaled up to 100μL to generate 10μg of cDNA from single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.
- Linear target amplification for real-time PCR
- Higher yields and precision than other cDNA synthesis kits at a fraction of the cost
- Ten-fold greater dynamic range than other kits
- Optimal reaction temperature: 37°C
- Final product size: 7kb or less
- Extensively tested with a variety of templates
- Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA
PCR & Real-Time PCR, Reverse Transcription
Order Info
Shipping Condition: Dry ice
Specifications
50 U/μL | |
First-Strand cDNA | |
RT-PCR, RT-qPCR | |
120 min. | |
RNA | |
100 μL, 20 μL |
Primer-Probe | |
First-Strand cDNA Synthesis Kit | |
Separate components | |
Reverse Transcription | |
Reverse Transcription | |
Yes |
For Research Use Only. Not for use in diagnostic procedures.
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