Applied Biosystems™ Protein Thermal Shift™ Starter Kit

This solution significantly reduces the time and cost associated with screening samples relative to currently available methods.

Supplier:  Applied Biosystems™ 4462263

Catalog No. 44-622-63

Description

Applied Biosystems™ Protein Thermal Shift™ kits allow users to employ a dye that binds to exposed hydrophobic residues to monitor the thermal stability of proteins using a real-time PCR instrument in order to identify buffer conditions that stabilize the protein of interest or screen libraries of ligands for compounds that bind the protein of interest.

Save Time and Money with Sample Screening: This solution significantly reduces the time and cost associated with screening samples relative to currently available methods.

Two Kits to Choose From: The Protein Thermal Shift™ Dye Kit contains the dye and buffer for performing protein thermal shift experiments, enough for up to 1000 reactions. The Protein Thermal Shift™ Starter Kit contains the Dye Kit plus a control protein and control ligand for test experiments and troubleshooting. The Protein Thermal Shift™ Starter Kit components are packaged in two boxes, one to be stored at -20°C and the other at 4°C, for maximum flexibility in reagent formulation.

Simplify Analysis with Protein Thermal Shift™ Software: Our complete Protein Thermal Shift™ solution includes analysis software which is compatible with run files generated from five of our real-time PCR systems: StepOne™ Real-Time PCR System, StepOnePlus™ Real-Time PCR system, 7500 Fast Real-Time PCR System, and ViiA™7 Real-Time PCR System.

• Screen samples for relative protein thermal stability
• Screen molecules (e.g. small molecule drug candidates, fragment-based libraries, and antibodies) for ligand binding to proteins in a high-throughput manner
• Screen samples for stability changes after protein point mutations or modifications
• Systematically identify suitable/optimal buffer conditions for the measurement of protein-ligand interactions, production of stable protein preparations, protein crystallization, and storage of proteins
• Quickly QC protein preps to ensure protein integrity and/or purity

Specifications

• Content And Storage: Store dye and buffer at 18° to 25°C
  Store control ligand and control protein at -15° to -25°C.
• PCR Method: qPCR
• For Use With (Equipment): 7500 Fast System, 7500 System, StepOne™, StepOnePlus™, ViiA™ 7 System
• Product Line: Protein Thermal Shift™
• Target Specificity: Not Target-Specific
• No. of Reactions: 1,000 reactions

• Detection Method: Primer-probe
• Technique: Fluorescence Intensity
• Kit Type: Starter Kit
• Quantity: 1,000 reactions
• For Use With (Application): Protein Thermal Shift
• Sample Type: Protein

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.