Applied Biosystems™ TaqMan™ Reverse Transcription Reagents

For use with reverse transcriptase-PCR (RT-PCR)

Includes: MultiScribe™ Reverse Transcriptase (100μL at 50U/μL)

Catalog Number	Mfr. No.	No. of Reactions
N8080234	Applied Biosystems™ N8080234	200 Reactions
43-041-34	Applied Biosystems™ 4304134	2,000 Reactions

Description

Random hexamers, oligo (dT)₁₆, and reverse primers are included for cDNA synthesis flexibility.

• Contain MultiScribe Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT)
• Random hexamers and oligo (dT)₁₆ included for flexibility
• Individual components provide versatility in assay set-up
• Compatible with one-step or two-step RT-PCR reactions
• Optimal reaction temperature: 42°C
• Final product size: 5kb or less

Using TaqMan Reverse Transcription Reagents TaqMan Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA. For two-step RT-PCR, random hexamers, oligo (dT)₁₆, and sequence-specific reverse primers can all be used. The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems.

Built-in normalization The TaqMan reverse transcription reagents contain a passive internal reference to normalize non-PCR-related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube.

Applications:
PCR & Real-Time PCR, Reverse Transcription

Specifications

• Concentration: 50 U/μL
• Format: Reverse Transcription Reagent
• Reaction Format: Separate components
• Size (Final Product): Up to 5 kb
• Technique: Reverse Transcription
• Reverse Transcriptase: MultiScribe

• Final Product Type: First-Strand cDNA
• PCR Method: RT-PCR, RT-qPCR
• Reagent Type: Reverse Transcription
• Starting Material: RNA
• Optimal Reaction Temperature: 42°C
• Ribonuclease H Activity: Yes

For Research Use Only. Not for use in diagnostic procedures.