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Applied Biosystems™ TaqMan™ Reverse Transcription Reagents
TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.
$834.65 - $8334.65
Specifications
Format | Kit |
---|---|
Technique | Reverse Transcription |
Optimal Reaction Temperature | 42°C |
Reverse Transcriptase | MultiScribe |
Ribonuclease H Activity | Yes |
Includes: MultiScribe™ Reverse Transcriptase (100μL at 50U/μL)
Catalog Number | Mfr. No. | No. of Reactions | Price | Quantity & Availability | |||||
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Catalog Number | Mfr. No. | No. of Reactions | Price | Quantity & Availability | |||||
N8080234
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Applied Biosystems™
N8080234 |
200 Reactions at 10 μL |
Each
for $834.65
Save 11% Reg : |
|
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43-041-34
![]() |
Applied Biosystems™
4304134 |
2000 Reactions at 10 μL |
Each
for $8,334.65
Save 7% Reg : |
|
|||||
Description
TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility. These reagents are designed for reverse transcription and PCR amplification of a specific target RNA from either total RNA or mRNA. The reagents in this kit facilitate two-step RT-PCR, which is performed in two separate reactions and is useful when detecting multiple transcripts from a single cDNA reaction or when storing a portion of the cDNA.
Advantages of TaqMan Reverse Transcription Reagents
- Contains MultiScribe Reverse Transcriptase, a recombinant Moloney murine leukemia virus (MMLV) reverse transcriptase
- Includes random hexamers and oligo (dT)16 for flexibility
- Individual components provide versatility in assay setup
- Compatible with two-step RT-PCR reactions
Using TaqMan Reverse Transcription Reagents
- TaqMan Reverse Transcription Reagents provide all the necessary components for reverse transcription of RNA to cDNA
- For two-step RT-PCR, random hexamers, oligo (dT)16, and sequence-specific reverse primers can all be used
- The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems
Specifications
Kit | |
42°C | |
Yes | |
Real Time PCR (qPCR) | |
First-Strand cDNA | |
Reverse Transcription | |
RNA |
Reverse Transcription | |
MultiScribe | |
Dry Ice | |
50 U/μL | |
Separate components | |
Up to 5 kb |
For Research Use Only. Not for use in diagnostic procedures.
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