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Applied Biosystems™ TaqMan™ Reverse Transcription Reagents
For use with reverse transcriptase-PCR (RT-PCR)
$764.00 - $7370.00
Specifications
Concentration | 50 U/μL |
---|---|
Final Product Type | First-Strand cDNA |
Format | Reverse Transcription Reagent |
PCR Method | RT-PCR, RT-qPCR |
Reaction Format | Separate components |
Includes: MultiScribe™ Reverse Transcriptase (100μL at 50U/μL)
Description
Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.- Contain MultiScribe Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT)
- Random hexamers and oligo (dT)16 included for flexibility
- Individual components provide versatility in assay set-up
- Compatible with one-step or two-step RT-PCR reactions
- Optimal reaction temperature: 42°C
- Final product size: 5kb or less
Using TaqMan Reverse Transcription Reagents TaqMan Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA. For two-step RT-PCR, random hexamers, oligo (dT)16, and sequence-specific reverse primers can all be used. The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems.
Built-in normalization The TaqMan reverse transcription reagents contain a passive internal reference to normalize non-PCR-related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube.
Applications:
PCR & Real-Time PCR, Reverse Transcription
Specifications
50 U/μL | |
Reverse Transcription Reagent | |
Separate components | |
Up to 5 kb | |
Reverse Transcription | |
MultiScribe |
First-Strand cDNA | |
RT-PCR, RT-qPCR | |
Reverse Transcription | |
RNA | |
42°C | |
Yes |
For Research Use Only. Not for use in diagnostic procedures.
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