R&D Systems™ AQUApure Tri-Ub Chains (K11-linked), Carrier-Free Protein

Catalog No. UC50025

Supplier: R&D Systems™ UC50025

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The AQUApure Tri-Ub Chains (K11-linked) Protein is derived from E. coli. The AQUApure Tri-Ub Chains (K11-linked) Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: P0CG47
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 7314
• Molecular Weight (g/mol): Theoretical M.W.: 26kDa
• Quantity: 25 μg
• Source: E. coli-derived human Tri-Ubiquitin protein
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 3 months, -20 to -70° C under sterile conditions after opening.
• Common Name: Tri-Ubiquitin
• Gene Symbol: UBB
• Biological Activity: Ubiquitin chains vary in length, linkage, and function. K11-linked Tri-Ubiquitin Chains (Ub3) are ideal for investigating Ubiquitin-binding proteins and as substrates for Ubiquitin-specific isopeptidases. Reaction conditions will need to be optimized for each specific application. IMPORTANT: Heating this product in SDS-PAGE buffer or terminating reactions containing this product with heated SDS-PAGE buffer could lead to unexpected, high apparent molecular weight banding or smearing on gels that is not representative of product purity. For optimal results, we recommend incubation in SDS-PAGE buffer + DTT at <40°C for 20 minutes prior to gel electrophoresis.
• Conjugate: Unconjugated
• Species: Human
• Expression System: E. coli-derived
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain. Ub-AQUA analysis: K11: 99.14% K63: 0.52% K6 : 0.15% K33: 0.12% All other linkages ≤ 0.03%