Molecular Probes ARES Alexa Fluor 647 DNA Labeling Kit
ARES Alexa Fluor 647 DNA Labeling Kit
Manufacturer: Molecular Probes A21676
DescriptionThe ARES Alexa Fluor DNA Labeling Kit provides a versatile, two-step method for labeling DNA with our Alexa Fluor dyes. In the first step, an amine-modified nucleotide is incorporated into DNA using conventional enzymatic labeling methods. In the second step, the amine-modified DNA is chemically labeled using our proprietary amine-reactive Alexa Fluor 647 dye. The labeled probes can be used for fluorescence in situ hybridization (FISH) and microarray techniques. We offer ARES Alexa Fluor DNA Labeling Kits in five fluorescent colors, and each kit provides sufficient reagents for 5 to 10 labeling reactions of 1 to 5µg DNA each.
ARES Alexa Fluor Labeling Kit Specifications:
More Uniform Labeling With ARES Labeling Kits
ARES Alexa Fluor Labeling Kits employ a two-step labeling technologynick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor dyes. This method achieves uniformity and consistency of labeling that is difficult to obtain with conventional enzymatic incorporation of labeled nucleotides.
We also offer this two-step labeling technology in our FISH Tag DNA and FISH Tag RNA Kits, which provide a complete workflow solution for FISH applications, including all of the reagents for probe synthesis, labeling, purification and an anti-fade reagent to help protect the signal during fluorescence microscopy.
More Options for Nucleic Acid Labeling
To review various options for nucleic acid labeling (including DNA and RNA FISH), review Labeling Oligonucleotides and Nucleic AcidsSection 8.2 in the Molecular Probes Handbook.
For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
|ARES™ Alexa Fluor™ 647 DNA Labeling Kit, Fluorescent Detection Method, Alexa Fluor™ 647 Dye, DNA (General), cDNA Labeling Target, Store at –5 to –30°C, Used For Labeling Chemistry, Microarray Analysis, Nucleic Acid Labeling and Oligo Synthesis|
|Alexa Fluor™ 647|
|DNA & RNA purification & analysis, cell analysis, labeling chemistry, labeling amines, amine-reactive fluorophores, nucleic acid chemical labeling, nucleic acid labeling & oligo synthesis, DNA labeling, gene expression analysis & genotyping, microarray analysis, expression array labeling|
|50μL 5-(3-aminoallyl)-dUTP (component A), 10 vials reactive dye (component B), 200μL Dimethylsulfoxide (DMSO) or dimethylformamide (DMF) (component C), 84mg Sodium bicarbonate (MW = 84.0) (component D), 10mL nuclease-free water (component E)|
|DNA (general), cDNA|
|Probes (Labeled DNA), cDNA (Labeled)|
|ARES™ Alexa Fluor™ 647 DNA Labeling Kit|
|–5 to –30°C|
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For Research Use Only. Not for use in diagnostic procedures.