Invitrogen™ ARF1/ARF3/ARF5/ARF6 Monoclonal Antibody (1D9)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA3060

Catalog No. MA3060

Description

MA3-060 detects ADP-ribosylation factor1 (Arf1), Arf3, Arf5 and Arf6 and about ten-fold less well with Arf4 from human, mouse and rat tissues. MA3-060 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects a single ~21 kDa protein representing Arf in rat pancreas extract. Immunofluorescence staining of Arf in rat pancreas with MA3-060 results in staining of the cytoplasmic face of the trans-Golgi membranes. MA3-060 cannot be used to neutralize Arf in solution. The MA3-060 antigen is recombinant human Arf1.

ADP-ribosylation factor 1 (ARF1) is a member of the humanARFgene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6ARFproteins and 11ARF-like proteins, constitute a family of the RAS superfamily. TheARFproteins are categorized as class I (ARF1,ARF2 andARF3), class II (ARF4 andARF5) and class III (ARF6), and members of each class share a common gene organization. TheARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008].

Specifications

• Antigen: ARF1/ARF3/ARF5/ARF6
• Classification: Monoclonal
• Concentration: Conc. Not Determined
• Formulation: Ascites with 0.05% sodium azide
• Gene Accession No.: P18085, P61204, P61205, P61206, P61750, P61751, P62330, P62331, P62332, P84077, P84078, P84079, P84082, P84083, P84084, P84085, Q8BSL7
• Gene Symbols: ARF1, Arf2, ARF3, Arf4, ARF5, ARF6
• Immunogen: Recombinant human ARF1.
• Regulatory Status: RUO
• Gene ID (Entrez): 100757864, 100759676, 100766631, 100769922, 100774654, 100856517, 11840, 11841, 11842, 11843, 11844, 11845, 140940, 375, 377, 378, 381, 382, 475206, 486562, 490679, 607073, 64310, 79117, 79119, 79120, 79121
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: 1D9
• Conjugate: Unconjugated
• Gene: Arf4
• Gene Alias: 5430400P17Rik; AA407803; Ac1-253; ADP ribosylation factor 1; ADP ribosylation factor 3; ADP ribosylation factor 4; ADP ribosylation factor 5; ADP ribosylation factor 6; ADP-ribosylation factor 1; ADP-ribosylation factor 2; ADP-ribosylation factor 3; ADP-ribosylation factor 4; ADP-ribosylation factor 4-like protein; ADP-ribosylation factor 5; ADP-ribosylation factor 6; AI788669; AI854770; ARF; Arf1; Arf2; ARF3; ARF4; Arf5; ARF6; AW496366; I79_003112; Liver regeneration-related protein LRRG202; pan ARF; small GTP binding protein
• Host Species: Mouse
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Canine, Hamster
• Product Type: Antibody
• Isotype: IgG1