Invitrogen™ Armenian Hamster IgG Isotype Control (eBio299Arm), Alexa Fluor™ 532, eBioscience™

Catalog No.	Quantity
58-488-880	25 μg

Catalog No. 58-488-880 Supplier Invitrogen™ Supplier No. 58488880

Description

Armenian Hamster Monoclonal Isotype Control

The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin. Applications Reported: This eBio299Arm antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio299Arm antibody has been tested by flow cytometric analysis of mouse splenocytes and human peripheral blood cells. This should be used at the same concentration as the experimental antibody. Alexa Fluor 532 is excited with the Green laser (532 nm) and emits at 561 nm. This cannot be used with the Yellow-Green laser (561 nm). We recommend using a 560/14 band pass filter. Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser.

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Specifications

• Antigen: Armenian Hamster IgG
• Applications: Control, Flow Cytometry
• Classification: Monoclonal
• Clone: eBio299Arm
• Concentration: 0.2 mg/mL
• Conjugate: Alexa Fluor 532
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: IgG; Immunoglobulin G; ImmunoglobulinG
• Host Species: Armenian Hamster
• Purification Method: Affinity chromatography
• Quantity: 25 μg
• Regulatory Status: RUO
• Primary or Secondary: Isotype Control (Primary)
• Target Species: Not Applicable
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Isotype Control
• Form: Liquid
• Isotype: IgG

Safety and Handling

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