Invitrogen™ Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™, Invitrogen™

Catalog No.	Quantity
50-112-9592	500 μg
50-145-45	50 μg

Catalog No. 50-112-9592 Supplier Invitrogen™ Supplier No. 16488885

Description

Armenian Hamster Isotype Control

Description: The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin. Applications Reported: The eBio299Arm antibody has been reported for use as an isotype control for flow cytometry. Applications Tested: This eBio299Arm antibody has been tested by LAL assay to determine endotoxin level and by immunofluorescent staining and flow cytometric analysis of mouse splenocytes and normal human peripheral blood. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Specifications

• Antigen: Armenian Hamster IgG
• Applications: Control, Flow Cytometry, Functional Assay
• Classification: Monoclonal
• Clone: eBio299Arm
• Concentration: 1 mg/mL
• Conjugate: Functional Grade
• Formulation: PBS with no preservative; pH 7.2
• Gene Alias: IgG; Immunoglobulin G; ImmunoglobulinG
• Host Species: Armenian Hamster
• Purification Method: Affinity chromatography
• Quantity: 500 μg
• Regulatory Status: RUO
• Primary or Secondary: Isotype Control (Primary)
• Target Species: Not Applicable
• Content And Storage: 4°C
• Product Type: Isotype Control
• Form: Liquid
• Isotype: IgG

For Research Use Only.