Invitrogen™ ATP Synthase beta Monoclonal Antibody (3D5AB1)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ A21351

Catalog No. A21351

Description

The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Near homogeneity was judged by SDS-PAGE. The epitope recognized by the anti-ATP synthase beta 3D5AB1 is in the region containing the active site of the beta-subunit and is centered approximately at amino acid residue 83. The complete amino acid sequence of the epitope is not known. See Chi SL, Wahl ML, Kenan DJ et al. (2007) Angiostatin-like activity of a monoclonal antibody to the catalytic subunit of F1F0 ATP synthase. Cancer Res 67:4716-4724.

ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.

Specifications

• Antigen: ATP Synthase beta
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: HEPES buffered saline with 0.02% sodium azide
• Gene Accession No.: P00829, P06576, P10719, P56480
• Gene Symbols: ATP5F1B
• Immunogen: Human Heart Mitochondria.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Monkey, Bovine, C. elegans
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Western Blot, Immunocytochemistry
• Clone: 3D5AB1
• Conjugate: Unconjugated
• Gene: ATP5F1B
• Gene Alias: ATP synthase F1 subunit beta; ATP synthase subunit beta, mitochondrial; ATP synthase, H+ transporting mitochondrial F1 complex, alpha subunit; ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit; ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide; Atp5b; ATP5F1B; ATPMB; ATPSB; beta-subunit; epididymis secretory protein Li 271; f1-ATPase beta; F1-ATPase beta-subunit; F-1-ATPase beta-subunit precursor; fj13e04; fj55c09; HEL-S-271; hm:zehn0534; hypothetical protein LOC554135; im:6793121; MGC5231; mitochondrial ATP synthase beta subunit; mitochondrial ATP synthase subunit beta subunit; mitochondrial ATP synthase, H+ transporting F1 complex beta subunit; mitochondrial ATP synthetase, beta subunit; wu:fj13e04; wu:fj38d01; wu:fj55c09; zgc:111961
• Host Species: Mouse
• Purification Method: purified
• Regulatory Status: RUO
• Gene ID (Entrez): 11947, 171374, 327675, 506
• Content And Storage: 4°C
• Form: Liquid