Invitrogen™ ATP7A Monoclonal Antibody (L60/4)

Catalog No.	Quantity
PIMA527720	100 μg

Catalog No. PIMA527720 Supplier Invitrogen™ Supplier No. MA527720

Description

Mouse Monoclonal Antibody

1 μg/mL of MA5-27720 was sufficient for detection of Copper-transporting ATPase1 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat IgG:HRP as the secondary antibody. Detects approximately 180kDa in rat brain membrane preparations. This antibody was formerly sold as clone S60-4.

ATP7A (also known as Copper-transporting ATPase 1) functions as a transmembrane copper-translocating P-type ATPase and plays a vital role in systemic copper absorption in the gut and copper reabsorption in the kidney. Polarized epithelial cells such as Madin-Darby canine kidney cells are a physiologically relevant model for systemic copper absorption and reabsorption in vivo. Although ATP7A is not detectable in most normal tissues, it is expressed in a considerable fraction of many common tumor types. Increased expression of ATP7A renders cells resistant to cisplatin and carboplatin. Mutations in the ATP7A gene result in Menkes disease, which is fatal in early childhood.

Specifications

• Antigen: ATP7A
• Applications: Immunohistochemistry (PFA fixed), Immunoprecipitation, Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: L60/4
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 50% glycerol and 0.09% sodium azide; pH 7.4
• Gene: ATP7A
• Gene Accession No.: P70705, Q04656, Q64430
• Gene Alias: ATP 7A; ATP7A; ATPase copper transporting alpha; ATPase, Cu++ transporting, alpha polypeptide; copper pump 1; Copper-transporting ATPase 1; Cu++-transporting P-type ATPase; DSMAX; FLJ17790; MC 1; MC1; Menke; Menkes disease-associated protein; menkes disease-associated protein homolog; Menkes protein; Menkes syndrome; MK; MNK; OHS; OTTHUMP00000062077; SMAX3
• Gene Symbols: ATP7A
• Host Species: Mouse
• Immunogen: Synthetic peptide amino acids 42-61 (cytoplasmic C-terminus) of human Copper- transporting ATPase1.
• Purification Method: Protein G
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 11977, 24941, 538
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b