B7-H1/PD-L1/CD274 Rat anti-Mouse, Alexa Fluor 594, Clone: 929903, R&D Systems™

Rat Monoclonal Antibody

Manufacturer:  R&D Systems FAB9078T025

Catalog No. FAB9078T025

Description

Mouse B7 homolog 1(B7-H1), also called programmed death ligand 1 (PD-L1) and programmed cell death 1 ligand 1 (PDCD1L1), is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1-4). Other family members include B7-1, B7-2, B7-H2, B7-H3 and PD-L2. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and a short cytoplasmic region. Among the family members, they share from 20-40% amino acid (aa) sequence identity. The cloned mouse B7-H1/PD-L1 cDNA encodes a 290 aa type I membrane precursor protein with a putative 18 aa signal peptide, a 220 aa extracellular region containing one V-like and one C-like Ig domain, a 22 aa transmembrane region, and a 30 aa cytoplasmic domain. Mouse and human B7-H1/PD-L1 share approximately 70% aa sequence identity. B7-H1/PD-L1 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors. The other identified ligand is PD-L2. Mouse B7-H1/PD-L1 and PD-L2 share approximately 34% aa sequence identity and have similar functions. B7-H1/PD-L1 is constitutively expressed in various lymphoid and non-lymphoid organs including placenta, heart, pancreas, lung, liver, and endothelium (1-4). The expression of B7-H1/PD-L1 is detected on B cells, T cells, monocytes, dendritic cells and thymic epithelial cells. IFN-γ treatment induces B7-H1/PD-L1 expression in monocytes, dendritic cells, and endothelial cells. B7-H1/PD-L1 expression is also upregulated in a variety of tumor cell lines. On previously activated T cells, B7-H1/PD-L1 interaction with PD-1 inhibits TCR-mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. In contrast, a costimulatory function for the PD-1 ligands on resting T cells has also been reported (1-4).

Specifications

• Antigen: PD-L1/B7-H1
• Clone: 929903
• Dilution: Flow Cytometry 5 uL/10^6 cells
• Gene Accession No.: Q9EP73
• Gene Alias: B7-H, B7H1, B7-H1, B7H1PDCD1L1, CD274, CD274 antigenMGC142294, CD274 molecule, PDCD1L1, PDCD1LG1, PDCD1LG1MGC142296, PDL1, PD-L1, PD-L1B7 homolog 1, PDL1PDCD1 ligand 1, programmed cell death 1 ligand 1, Programmed death ligand 1
• Host Species: Rat
• Isotype: IgG2a
• Quantity: 25 Tests
• Storage Requirements: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
• Monoclonal or Polyclonal: Monoclonal
• Test Specificity: Detects mouse B7-H1/PD-L1 in direct ELISAs. Stains mouse B7-H1/PD-L1 transfectants but not irrelevant transfectants in flow cytometry.

• Applications: Flow Cytometry
• Conjugate: Alexa Fluor 594
• Formulation: Supplied in a saline solution containing BSA and Sodium Azide. with Sodium Azide
• Format: Purified
• Gene Symbols: Cd274
• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse B7-H1/PD-L1 Met1-Thr239 Accession # Q9EP73
• Purification Method: Protein A or G purified from hybridoma culture supernatant
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 29126
• Target Species: Mouse