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  5. Gibco™ Bacto™ CD Supreme Fermentation Production Medium (FPM)

Gibco™ Bacto™ CD Supreme Fermentation Production Medium (FPM)

Catalog No. A4973701
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10 kg
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Description

Gibco Bacto CD Supreme Fermentation Production Medium (FPM) is a chemically defined medium for microbial fermentation. It is a dehydrated powder medium (DPM) designed specifically for fermentation applications to support high-cell-density cultures of Escherichia coli.

  • Gibco Bacto CD Supreme Fermentation Production Medium (FPM) is a chemically defined medium for microbial fermentation
  • It is a dehydrated powder medium (DPM) designed specifically for fermentation applications to support high-cell-density cultures of Escherichia coli
  • Bacto CD Supreme FPM is chemically defined, hydrolysate-free, and animal origin component-free
  • It has not been in contact with material of animal origin during processing and therefore poses no TSE risk

Features of Bacto CD Supreme FPM include:

  • Reduce lot-to-lot performance variability with a 100% chemically defined formulation
  • Eliminate risk of bacteriophage and BSE/TSE contamination
  • Optimize your fermentation workflow by streamlining, reducing, or eliminating steps
  • Increase growth and production yields; shown to support growth of a variety of E. coli strains including DH10B, BL21(DE3), DH5α, DH1, Stbl2, Stbl3, One Shot TOP10, and others
  • Flexible sterilization methods allow the medium to be filter sterilized or autoclaved
  • Scalable format to meet your development needs

Specifications

Content And Storage 2°C to 8°C
Form Dehydrated Powder
Media Type Fermentation Production Medium
Product Type Dehydrated Culture Media
Fill Volume 500 g
For Use With (Application) Microbial Fermentation
Includes No antibiotics
Product Line Gibco, Bacto
Quantity 500 g
Strain Designations Escherichia coli

Frequently Asked Questions (FAQs)

Can I follow my typical IPTG induction process using Bacto CD Supreme Fermentation Production Medium (FPM)?

IPTG induction concentration and timing may need to be adjusted when using Bacto CD Supreme Fermentation Production Medium (FPM). Previous studies suggest that reduced IPTG concentration may improve results.
I purchased my E. coli strain from Thermo Fisher Scientific. Where can I find genotype information?

E. coli genotype information can be here (https://www.thermofisher.com/us/en/home/life-science/cloning/cloning-learning-center/invitrogen-school-of-molecular-biology/molecular-cloning/transformation/competent-cell-genotypes-genetic-markers.html).
How can I get regulatory documentation such as Drug Master Files (DMFs) for Bacto CD Supreme Fermentation Production Medium (FPM)?

Information regarding regulatory support can be found here (https://www.thermofisher.com/us/en/home/life-science/bioproduction/regulatory-support.html).

Drug Master Files (DMF) (USA Only): To have Thermo Fisher Scientific provide the FDA with an authorization letter to view our DMF, please use this form to send in the request (https://www.thermofisher.com/us/en/home/global/forms/life-science/dmf-authorization-letter-request.html).

For regions outside the USA, please use this form to request access to a confidential Regulatory Support File (https://www.thermofisher.com/us/en/home/global/forms/life-science/dmf-authorization-letter-request.html).
Can cells be banked directly using Bacto CD Supreme Fermentation Production Medium (FPM)?

Yes, cells can be banked using Bacto CD Supreme Fermentation Production Medium (FPM).
How is Bacto CD Supreme Fermentation Production Medium (FPM) different from traditional microbial media or hydrolysates?

Bacto CD Supreme Fermentation Production Medium (FPM) is chemically-defined and can be sterilized using membrane filtration or autoclaved.
Are there any components in Bacto CD Supreme Fermentation Production Medium (FPM) that are typically used as inducers?

No, there are no components present in Bacto CD Supreme Fermentation Production Medium (FPM) that are typically used as inducers.
How can I resolve excessive lag phase (>5 hours) seen during bioreactor scale up with Bacto CD Supreme Fermentation Production Medium (FPM)?

We recommend performing your seed train with Bacto CD Supreme Fermentation Production Medium (FPM).
What carbon source is present in Bacto CD Supreme Fermentation Production Medium (FPM)?

There is no glucose or glycerol present in Bacto CD Supreme Fermentation Production Medium (FPM). The addition of carbon source must be made by the user. If autoclaving media components, be sure to autoclave carbon sources separate from Bacto CD Supreme FPM.
Can I use Bacto CD Supreme Fermentation Production Medium (FPM) for seed stock generation?

Bacto CD Supreme Fermentation Production Medium (FPM) can be used for seed stock generation. This is the recommended usage of Bacto CD Supreme FPM to ensure optimal results.
I'm interested in achieving faster growth. How can I ensure successful transition from LB Broth to Bacto CD Supreme Fermentation Production Medium (FPM)?

To achieve faster growth, additional glucose may be added at 2-5 g/L to the glycerol-containing Bacto CD Supreme Fermentation Production Medium (FPM).
Is a carbon source required for use with Bacto CD Supreme Fermentation Production Medium (FPM)?

Yes, glycerol and/or glucose are required for use with Bacto CD Supreme Fermentation Production Medium (FPM).

Is amino acid supplementation required for my E. coli strain when using Bacto CD Supreme Fermentation Production Medium (FPM)?

A detailed supplementation table can be found in the Bacto CD Supreme Fermentation Production Medium (FPM) user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0024960_BactoCDSupremeFPM_UG.pdf), for various E. coli strains. The genotype of your specific E. coli strain may also be provided by your vendor with supplementation guidance.

My E. coli strain is a derivative of DH10B or Top10 carrying delta (ara-leu) mutation. What supplementation do I need when using Bacto CD Supreme Fermentation Production Medium (FPM)?

A detailed supplementation table can be found in the Bacto CD Supreme Fermentation Production Medium (FPM) user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0024960_BactoCDSupremeFPM_UG.pdf), for various E. coli strains. L-leucine amino acid supplementation may be required, starting with a concentration of 0.5 g/L and optimized further.
Does Bacto CD Supreme Fermentation Production Medium (FPM) support the growth of other bacteria besides E. coli strains?

Bacto CD Supreme Fermentation Production Medium (FPM) has primarily been evaluated and developed using multiple E. coli strains. Bacto CD Supreme FPM may support the growth of other bacteria through additional studies and analysis by the customer with the bacteria species.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

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