Novus Biologicals™ BCAM/CD239 Antibody Pair [HRP]

Description

Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human BCAM/CD239.

Specifications

• Assay Range: 54.69 pg/mL
• Assay Sensitivity: 54.69-3500 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human BCAM/CD239.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: antigen identified by monoclonal F8, Auberger B antigen, basal cell adhesion molecule, basal cell adhesion molecule (Lu and Au blood groups), basal cell adhesion molecule (Lutheran blood group), B-CAM cell surface glycoprotein, B-cell adhesion molecule, CD239, CD239 antigen, F8/G253 antigen, glycoprotein 95kDa, LUAU, Lutheran antigen, Lutheran blood group (Auberger b antigen included), Lutheran blood group glycoprotein, MSK19
• Gene ID (Entrez): 4059
• Gene Symbol: BCAM
• Host Species: Rabbit/Mouse
• Kit Contents:
  Standard: Each vial contains 101 ng of recombinant Human BCAM/CD239.
  Capture Antibody: 1 mg/mL of mouse anti-Human BCAM/CD239 monoclonal antibody (in PBS, pH 7.4).
  Detection Antibody: 0.2 mg/mL of rabbit anti-Human BCAM/CD239 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Storage Requirements: Storage is content dependent.
• Target: BCAM/CD239
• Technique: 1. Capture Antibody: Dilute to a working concentration of 1 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.1 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3500 pg/mL is recommended.