Novus Biologicals™ Bcl-2 Antibody Pair [HRP]

Description

Mouse Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Bcl-2.

Specifications

• Assay Range: 78.13 pg/mL
• Assay Sensitivity: 78.13-5000 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Bcl-2.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, Bcl-2
• Gene ID (Entrez): 596
• Gene Symbol: BCL2
• Host Species: Mouse
• Kit Contents:
  Detection Antibody: 0.2 mg/mL of mouse anti-Human Bcl-2 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  Capture Antibody: 0.5 mg/mL of mouse anti-Human Bcl-2 monoclonal antibody (in PBS, pH 7.4).
  Standard: Each vial contains 252 ng of recombinant Human Bcl-2.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Apoptosis, Autophagy, Biologically Active Proteins, Cancer, Cellular Markers, Mitochondrial Mediated Pathway, Oncogenes, Phospho Specific, Tumor Suppressors
• Storage Requirements: Storage is content dependent.
• Target: Bcl-2
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5000 pg/mL is recommended.