Invitrogen™ Bcl-X Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA521676

Catalog No. PIPA521676

Description

Recommended positive controls: HCT116, NIH-3T3, PC-12, OKT3. Predicted reactivity: Mouse (97%), Rat (97%), Dog (100%), Cat (99%), Pig (97%), Rabbit (96%), Sheep (98%), Rhesus Monkey (99%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The protein encoded the BCL-X gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator.

Specifications

• Antigen: Bcl-X
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: BCL2L1
• Gene Alias: anti-apoptosis regulatory protein; anti-apoptotic Bcl-2 gene family member; anti-apoptotic Bcl-xL; anti-apoptotic regulator Bcl-xL; apoptosis regulator Bcl-X; B cell lymphoma 2 like; B cell lymphoma like X; B-cell leukemia/lymphoma x; Bcl 2 like 1; bcl x; Bcl xL; BCL XL/S; Bcl(X)L; bcl-2 L1; BCL2 like 1; BCL2L; Bcl2l1; bcl2-L-1; BCL2-like 1; Bcl-2-like protein 1; BCL2-like protein 1; BCLX; bcl-x; Bcl-x long protein; BclxL; Bcl-xL; BCL-XL/S; BclXS; bcl-xS; BLC2L; DKFZp781P2092; PPP1R52; protein phosphatase 1, regulatory subunit 52; RP5-857M17.3; unnamed protein product
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12048, 24888, 598
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 0.45 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: P53563, Q07817, Q64373
• Gene Symbols: BCL2L1
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 208 of Bcl-X (Uniprot ID#Q07817).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov