BD A431 + EGF Cell Lysate

Cell or tissue lysates are a useful control for the western blotting application

Manufacturer:  BD 611448

Catalog No. BDB611448

Includes: Human Epidermoid Carcinoma

Description

A source that can be rich in endogenously expressed proteins, this lysate is convenient to use and provided in a denatured format allowing for immediate loading onto an SDS-PAGE gel. A431 cells (Human epithelial carcinoma; ATCC CRL-1555) were grown to 80-90% confluency, serum starved overnight, then treated with 100ng/mL EGF for 5 minutes at 37°C.

• Storage buffer: SDS-PAGE buffer (62mM Tris pH 6.8, 2% SDS, 0.9% b-mercaptoethanol, 0.003% bromophenol blue, 5% glycerol)

Western blot

Specifications

• Tissue: Epidermoid Carcinoma
• Storage Buffer: 1mg/mL in SDS-PAGE Buffer: 62mM Tris pH 6.8, 2% SDS, 0.9% beta-mercaptoethanol, 0.003% Bromophenol Blue, 5% Glycerol
• Host Species: Human
• For Use With (Application): Western Blot

• Quantity: 500μg
• Storage Requirements: -20°C
• Concentration: 1mg/mL

Research Use Only