BD Ac-DEVD-CHO Inhibitor

Description

Requires

Apoptotic and non-apoptotic cells, phosphate buffered saline (PBS) Wash Buffer: 140mM NaCl, 2.7mM KCl, 10mM KH2PO4 dissolved in distilled, autoclaved water. Adjust the pH to 7.4 using hydrochloric acid, Cell Lysis Buffer: 10mM Tris (pH 7.5), 130mM NaCl, 1% Triton -X-100, 10mM NaPi, 10mM NaPPi, Caspase-3 (CPP32), Ac-DEVD-CHO Inhibitor, and substrate Ac-DEVD-AMC, Protease Assay Buffer: 20mM HEPES (pH 7.5), 10% glycerol, 2mM DTT

To identify and quantify Caspase-3 activity in apoptotic cells, and to study events downstream of Caspase-3 activation

Members of ICE/CED-3 cysteine protease family have key roles in inflammation and mammalian apoptosis. The ICE family member Caspase-3 (also known as CPP32, Yama, apopain) is activated early in apoptosis and appears to be involved in proteolysis of several important molecules, including poly (ADP ribose) polymerase (PARP)

• Synthetic tetrapeptide inhibitor for Caspase-3
• Used in protease assays
• Cleaves PARP from 116kD to 85kD residual fragment
• Cleavage site in PARP is C-terminal to Asp-216
• Upstream sequence of cleavage site, DEVD (Asp-Glu-Val-Asp), is utilized as basis for highly specific Caspase-3 substrate, Ac-DEVD-AMC and Caspase-3 inhibitor,Ac-DEVD-CHO
• Storage Buffer: Lyophilized powder
• Format: Purified

Functional assay

Specifications

• For Use With (Application): Functional assay
• Storage Buffer: Lyophilized powder
• Content And Storage: -20°C
• Quantity: 1 mg
• Molecular Weight (g/mol): 502.5
• Inhibitors: Ac-DEVD-CHO
• Form: Purified