BD Caspase-3

Catalog No.	Concentration
BDB556472	0.2mg/mL

Catalog No. BDB556472 Supplier BD Supplier No. 556472

Description

Play key role in normal apoptosis during development induced by the death receptors

Caspases are cysteine proteases that play central role in apoptosis. The caspase family was discovered by searching human cDNA libraries for sequences homologous to ced-3, a C. Each caspase is synthesized as an inactive proenzyme that is processed by cleavage at asparte residues by another protease or by self-proteolysis.

• Fas and tumor necrosis factor receptor type 1 (TNFR1)
• One of their substrates is poly (ADP ribose) polymerase (PARP)
• Cleave PARP from its 116kDa form to 85kDa residual fragment
• The cleavage site in PARP is C-terminal to Asp-216
• Utilized as basis for highly specific caspase-3 substrates such as Ac(N-acetyl)-DEVD-AFC (7-amino-4-trifluoromethylcourmarin) and Ac(N-acetyl)-DEVD-AMC (7-amino-4-methylcoumarin) as well as caspase-3 aldehyde inhibitor Ac-DEVD-CHO
• Storage Buffer: 50mM Tris (pH 8.0) with 100mM NaCl and 50mM imidazole
• Format: Purified

Specifications

• Concentration: 0.2mg/mL
• For Use With (Application): Enzyme Assay
• Formulation: 50mM Tris (pH 8) with 100mM NaCl and 50mM Imidazole
• Name: Human Caspase-3 Active Form Recombinant Protein
• Quantity: 5μg
• Storage Requirements: -80°C
• Cross Reactivity: Human
• Recombinant: Recombinant
• Form: Purified

Research use only