BD Caspase-3

Play key role in normal apoptosis during development induced by the death receptors

Supplier:  BD 556472

Catalog No. BDB556472

Description

Caspases are cysteine proteases that play central role in apoptosis. The caspase family was discovered by searching human cDNA libraries for sequences homologous to ced-3, a C. Each caspase is synthesized as an inactive proenzyme that is processed by cleavage at asparte residues by another protease or by self-proteolysis.

• Fas and tumor necrosis factor receptor type 1 (TNFR1)
• One of their substrates is poly (ADP ribose) polymerase (PARP)
• Cleave PARP from its 116kDa form to 85kDa residual fragment
• The cleavage site in PARP is C-terminal to Asp-216
• Utilized as basis for highly specific caspase-3 substrates such as Ac(N-acetyl)-DEVD-AFC (7-amino-4-trifluoromethylcourmarin) and Ac(N-acetyl)-DEVD-AMC (7-amino-4-methylcoumarin) as well as caspase-3 aldehyde inhibitor Ac-DEVD-CHO
• Storage Buffer: 50mM Tris (pH 8.0) with 100mM NaCl and 50mM imidazole
• Format: Purified

Specifications

• Concentration: 0.2mg/mL
• Formulation: 50mM Tris (pH 8) with 100mM NaCl and 50mM Imidazole
• Quantity: 5μg
• Cross Reactivity: Human
• Form: Purified

• For Use With (Application): Enzyme Assay
• Name: Human Caspase-3 Active Form Recombinant Protein
• Storage Requirements: -80°C
• Recombinant: Recombinant

Research use only