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BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution

1X solution can be used for the simultaneous fixation and permeabilization of cells prior to intracellular cytokine staining

Manufacturer:  BD 554722

Catalog No. BDB554722

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Recommended Assay Procedure: For the stimulation of cells, various in vitro methods have been reported for stimulating cells to produce cytokines. Polyclonal activators have been particularly useful for inducing cytokine-producing cells. These activators include the following: concanavalin A, lipopolysaccharide, phorbol esters plus calcium ionophore or ionomycin, phytohaemaglutinin, staphylococcus enterotoxin B, and monoclonal antibodies directed against subunits of the TCR/CD3 complex (with or without antibodies directed against costimulatory receptors, such as CD28).


Can be used for the simultaneous fixation and permeabilization of cells prior to intracellular Cyto kine staining
Store undiluted at 4°C
Fixation and permeabilization solution
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