BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution

Catalog No. BDB554722

Supplier: BD 554722

Description

1X solution can be used for the simultaneous fixation and permeabilization of cells prior to intracellular cytokine staining

Recommended Assay Procedure:
For the stimulation of cells, various in vitro methods have been reported for stimulating cells to produce cytokines. Polyclonal activators have been particularly useful for inducing cytokine-producing cells. These activators include the following: concanavalin A, lipopolysaccharide, phorbol esters plus calcium ionophore or ionomycin, phytohaemaglutinin, staphylococcus enterotoxin B, and monoclonal antibodies directed against subunits of the TCR/CD3 complex (with or without antibodies directed against costimulatory receptors, such as CD28).

Specifications

• For Use With (Application): Can be used for the simultaneous fixation and permeabilization of cells prior to intracellular Cyto kine staining
• pH Range: 7.2
• Content And Storage: Store undiluted at 4°C
• Product Type: Fixation and permeabilization solution
• Quantity: 125 mL
• Format: Liquid