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BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution
1X solution can be used for the simultaneous fixation and permeabilization of cells prior to intracellular cytokine staining
Manufacturer: BD 554722
Description
Recommended Assay Procedure: For the stimulation of cells, various in vitro methods have been reported for stimulating cells to produce cytokines. Polyclonal activators have been particularly useful for inducing cytokine-producing cells. These activators include the following: concanavalin A, lipopolysaccharide, phorbol esters plus calcium ionophore or ionomycin, phytohaemaglutinin, staphylococcus enterotoxin B, and monoclonal antibodies directed against subunits of the TCR/CD3 complex (with or without antibodies directed against costimulatory receptors, such as CD28).
Specifications
| Can be used for the simultaneous fixation and permeabilization of cells prior to intracellular Cyto kine staining | |
| Store undiluted at 4°C | |
| 125mL |
| 7.2 | |
| Fixation and permeabilization solution | |
| Liquid |
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