Protein Assays and Amino Acid Analysis
Protein Assays and Amino Acid Analysis
Block the intracellular protein transport processes when added to in vitro- or in vivo-stimulated lymphoid cells
Can be used in intracellular cytokine staining to permeabilize cells and as an antibody diluent and cell wash buffer
1X solution can be used for the simultaneous fixation and permeabilization of cells prior to intracellular cytokine staining
Enhance the detectability of cytokine-producing cells with immunofluorescent staining and flow cytometric analysis
Serves as potent, selective, competitive, high affinity inhibitor of arginase.
|Content And Storage||−20°C|
|Description||Purity: ≥97% by TLC|
|Product Type||Nω-Hydroxy-nor-L-arginine, Diacetate Salt, Packaged in Glass Bottle|
|For Use With (Application)||Arginase from rat liver|
Serves as key intermediate in biosynsis of nitric oxide by constitutive nitric oxide synthase.
Use for Controlling background noise. Cytiva Life Sciences™ Amersham™ ECL Advance™ is Blocking Reagent controls background noise while retaining a high level of signal intensity.
Use for accurate determination of protein concentration in samples prepared for electrophoresis techniques. Cytiva Life Sciences™ 2-D Quant Kit precipitates proteins while leaving interfering substances behind.
|Includes||Plate coating Antibody, in vivo capture Antibody, IVC standard, Enzyme concentrate, technical data sheet and lot specific certificate of analysis|
Can be used to fix unstained cells for subsequent immunofluorescent staining of intracellular cytokines. Can also be used to fix cells after immunofluorescent staining in order to preserve the light-scattering signals and fluorescent intensities of cells for analysis at a later time.
SDS-PAGE, western blot, Color protein standard
Spectrophotometric assay for the quantitation of glutathione peroxidase in cells, erythrocytes, blood, and tissues
Designed for in vitro MuRF1-mediated ubiquitination of user-supplied substrates such as cardiac troponin 1, MYH7, or muscle creatine kinase
Designed for the in vitro ubiquitination of p53 by MDM2 with subsequent Western blot
Provides a functional in vitro HSP70/HSP40 refolding system with included substrate protein or user-supplied proteins
Designed for in vitro ubiquitination of user-supplied substrates by CHIP or the CHIP/HSP70/HSP40 ternary complex
Provides a functional in vitro HSP60/HSP10 refolding system with included substrate protein or user-supplied proteins
Rapid and economical method to identify E2 enzyme(s) that functionally interact with an E3 ubiquitin ligase in vitro
Designed for the conjugation of the ubiquitin-like modifier NEDD8 to protein substrates in vitro
This kit produces His-fusion proteins at high purities. The wheat germ included in this kit is a specialized wheat germ extract prepared by removing endogenous proteins as nonspecifically adsorb to His-tag affinity purification resin.
This kit produces GST-fusion proteins at high purities and high yields. The wheat germ included in this kit is a specialized high yield wheat germ extract prepared by removing endogenous proteins as nonspecifically adsorb to GST-tag affinity purification resin.
Arginase Assay Kit is a quantitative colorimetric arginase determination.
Ketone Body Assay Kit is a quantitative colorimetric determination of ketone body at 340 nm.
General Gene Knockdown Quantification Kit is used for measuring gene knockdown induced by siRNA or antisense oligonucleotide at the protein level.
Recombinant full-length human PDK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. PDK1 (3-phosphoinositide-dependent protein kinase) is activated by the presence of PtdIns(3,4,5)P3 or PtdIns(3,4)P2.
|Includes||10μg Recombinant PDK1 Kinase, PDKtide (KTFCGTPEYLAPEVRREPRILSEEEQEMFRDFDYIADWC) Substrate, Reaction Buffer, DTT and MnCl2|
|Product Type||PDK1 Kinase Enzyme system|
MEK Inhibitor U0126 is a chemically synthesized organic compound that inhibits activation of MAPK (ERK 1/2) by inhibiting the kinase activity of MAP Kinase Kinase (MAPKK or MEK 1/2).
DNA-Dependent Protein Kinase Peptide Substrate is a highly specific substrate for DNA-PK, with the sequence Glu-Pro-Pro-Leu-Ser-Gln-Glu-Ala-Phe-Ala-Asp-Leu-Trp-Lys-Lys.
This kit produces His-fusion proteins at high purities and high yields. The wheat germ included in this kit is a specialized high yield wheat germ extract prepared by removing endogenous proteins as nonspecifically adsorb to His-tag affinity purification resin.