Protein Assays and Amino Acid Analysis
BD Cytofix™ Fixation Buffer
Can be used to fix unstained cells for subsequent immunofluorescent staining of intracellular cytokines. Can also be used to fix cells after immunofluorescent staining in order to preserve the light-scattering signals and fluorescent intensities of cells for analysis at a later time.
BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution
1X solution can be used for the simultaneous fixation and permeabilization of cells prior to intracellular cytokine staining
MilliporeSigma™ mPAGE™ Color Protein Standard
SDS-PAGE, western blot, Color protein standard
Abnova™ Protein Expression Kit I (His-Fusion Protein)
This kit produces His-fusion proteins at high purities. The wheat germ included in this kit is a specialized wheat germ extract prepared by removing endogenous proteins as nonspecifically adsorb to His-tag affinity purification resin.
Abnova™ Protein Assay Kit
Protein Assay Kit is a bradford colorimetric protein determination at 595 nm.
BD GolgiPlug™ Protein Transport Inhibitor (containing Brefeldin A)
Enhance the detectability of cytokine-producing cells with immunofluorescent staining and flow cytometric analysis
BD Perm/Wash™ Buffer
Can be used in intracellular cytokine staining to permeabilize cells and as an antibody diluent and cell wash buffer
Invitrogen™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 1 Peptide
Z'-LYTE™ Kinase Assay Kit - Ser/Thr 1 Peptide
MilliporeSigma™ mPAGE™ Unstained Protein Standard
SDS-PAGE, western blot
Cytiva 2-D Quant Kit
Use for accurate determination of protein concentration in samples prepared for electrophoresis techniques. Cytiva Life Sciences™ 2-D Quant Kit precipitates proteins while leaving interfering substances behind.
MilliporeSigma™ Calbiochem™ NG-Hydroxy-L-arginine, Monoacetate Salt
Serves as key intermediate in biosynsis of nitric oxide by constitutive nitric oxide synthase.
BD GolgiStop™ Protein Transport Inhibitor (containing Monensin)
Block the intracellular protein transport processes when added to in vitro- or in vivo-stimulated lymphoid cells
Invitrogen™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 9 Peptide
Z'-LYTE Kinase Assay Kit - Ser/Thr 9 Peptide
R&D Systems™ High-Throughput Glutathione Peroxidase Assay Kit
Spectrophotometric assay for the quantitation of glutathione peroxidase in cells, erythrocytes, blood, and tissues