Protein Assays and Amino Acid Analysis
Protein Assays and Amino Acid Analysis
BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution
1X solution can be used for the simultaneous fixation and permeabilization of cells prior to intracellular cytokine staining
MilliporeSigma™ mPAGE™ Color Protein Standard
SDS-PAGE, western blot, Color protein standard
BD Perm/Wash™ Buffer
Can be used in intracellular cytokine staining to permeabilize cells and as an antibody diluent and cell wash buffer
Invitrogen™ Light Emission Accelerator-II
Light emission triggering solution with luminescence enhancer for use with the Galacto-Light Plus™ or Dual-Light™ Reporter Gene Assay Systems.
MilliporeSigma™ Calbiochem™ Nω-Hydroxy-nor-L-arginine, Diacetate Salt
Serves as potent, selective, competitive, high affinity inhibitor of arginase.
BD Cytofix™ Fixation Buffer
Can be used to fix unstained cells for subsequent immunofluorescent staining of intracellular cytokines. Can also be used to fix cells after immunofluorescent staining in order to preserve the light-scattering signals and fluorescent intensities of cells for analysis at a later time.
MilliporeSigma™ mPAGE™ Unstained Protein Standard
SDS-PAGE, western blot
MilliporeSigma™ Calbiochem™ NG-Hydroxy-L-arginine, Monoacetate Salt
Serves as key intermediate in biosynsis of nitric oxide by constitutive nitric oxide synthase.
BD BD GolgiStop™ Protein Transport Inhibitor (containing Monensin)
Block the intracellular protein transport processes when added to in vitro- or in vivo-stimulated lymphoid cells
BD GolgiPlug™ Protein Transport Inhibitor (containing Brefeldin A)
Enhance the detectability of cytokine-producing cells with immunofluorescent staining and flow cytometric analysis
Cytiva Amersham™ ECL Advance™ Blocking Reagent
Use for Controlling background noise. Cytiva Life Sciences™ Amersham™ ECL Advance™ is Blocking Reagent controls background noise while retaining a high level of signal intensity.
Cytiva 2-D Quant Kit
Use for accurate determination of protein concentration in samples prepared for electrophoresis techniques. Cytiva Life Sciences™ 2-D Quant Kit precipitates proteins while leaving interfering substances behind.
R&D Systems™ Human HSP60/HSP10 Protein Refolding Kit
Provides a functional in vitro HSP60/HSP10 refolding system with included substrate protein or user-supplied proteins
R&D Systems™ E2Select Ubiquitin Conjugation Kit
Rapid and economical method to identify E2 enzyme(s) that functionally interact with an E3 ubiquitin ligase in vitro