General methods in yeast genetics specify using yeast extract-peptone-dextrose (YPD) medium for cultivating Saccharomyces cerevisiae and other yeasts. Yeasts grow well on a minimal medium containing only dextrose and salts. The addition of protein and yeast cell extract hydrolysates allows faster growth so that during exponential or log-phase growth, the cells divide every 90 minutes.
- Peptone acts as a source of carbon, nitrogen, vitamins and minerals
- Yeast extract supplies B-complex vitamins, which stimulate bacterial growth
- Dextrose is the carbohydrate source
- Agar acts as the solidifying agent
- pH (6.5% solution, 25°C): 6.5 ± 0.2
Prices are for units of one each unless otherwise specified.
|For maintaining and propagating Yeasts in molecular microbiology procedures|
|Yeast extract 10g/L, Peptone 20g/L, Dextrose 20g/L, Agar 15g/L|
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