BD Horizon Brilliant Violet 480 Strepavidin
Streptavidin has very high binding affinity for biotin, which is conjugated to the antibody
Manufacturer: BD 564876
BD Horizon BV480 Streptavidin is a useful detection reagent for labeling biotinylated primary or secondary antibodies for detection of antigens by immunofluorescence.
Streptavidin was conjugated to BD Horizon BV480 which is part of the BD Horizon Brilliant Violet family of dyes. This dye has been exclusively developed by BD Biosciences as an optimal dye for imaging applications. BD Horizon BV480 has an Ex Max at 436nm and Em Max at 477nm. The use of a mounting reagent (eg, ProLong Gold) is highly recommended to maximize the photostability of BV480.
For confocal microscopy systems, a 440nm laser is the optimal excitation source with optimal emission collection centered at 477nm. BV480 fluorescence can also be excited well by 405nm and 458 nm lasers.
For epifluorescence microscopes with broad spectrum excitation sources, the recommended excitation and emission filters are 445/20nm and 485/20nm bandpass filters, respectively. For specific multicolor imaging applications, the exact filter configurations should be optimized by the end user.
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Aqueous buffered solution containing BSA and ≤0.09% sodium azide.|
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