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BD Jurkat + Pervanadate Lysate

For blotting procedures

Manufacturer:  BD 611755

Catalog No. BDB611755


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Includes: Human T-Cell Leukemia

Description

Description

  • Concentration: 1.0mg/mL
  • Rich in endogenously expressed proteins
  • T-Cells were grown to 80-90% confluency

Western blot

Specifications

Specifications

T-cell Leukemia
500μg
-20°C
1mg/mL
Jurkat (T-cell leukemia) cells, were grown to 80 to 90% confluency, then treated with 1mM pervanadate, a general inhibitor of Protein Tyrosine Phosphatases, for 15 min. at 37°C
1mg/mL in SDS-PAGE Buffer: 62mM Tris pH 6.8, 2% SDS, 0.9% beta-mercaptoethanol, 0.003% Bromophenol Blue, 5% Glycerol
Human
Western Blot
Documents
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