BD Mouse Macrophage plus IFNγ/LPS Lysate

Convenient to use and useful control for western blotting application

Manufacturer:  BD 611473

Catalog No. BDB611473

Description

• Source is rich in endogenously expressed proteins
• Provided in denatured format allowing for immediate loading onto SDS-PAGE gel
• Storage buffer: SDS-PAGE buffer (62mM Tris pH 6.8, 2% SDS, 0.9% b-mercaptoethanol, 0.003% bromophenol blue, 5% glycerol)

Routinely tested western blot

Specifications

• Tissue: Macrophage
• Quantity: 500μg
• Storage Requirements: -20°C
• Concentration: 1mg/mL

• Preparation Method: Mouse macrophages (RAW 264.7 cells) were grown to 70% confluencey, then stimulated with 10ng/mL IFNgamma and 1μg/mL LPS for 12 Hours, western blotting of mouse macrophages stimulated with IFNgamma and LPS
• Storage Buffer: 1mg/mL in SDS-PAGE Buffer: 62mM Tris pH 6.8, 2% SDS, 0.9% beta-mercaptoethanol, 0.003% Bromophenol Blue, 5% Glycerol
• Host Species: Mouse

Research use only