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BD NIH 3T3 + Vanadate + PDGF Lysate

Catalog No. BDB612069 Shop All BD Biosciences Products
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500 μg
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Catalog No. Quantity
BDB612069 500 μg
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Catalog No. BDB612069 Supplier BD Supplier No. 612069

Description

Includes

Mouse Embryonic Fibroblast

Useful control for the western blotting application

  • Can be rich in endogenously expressed proteins
  • Convenient to use and provided in a denatured format allowing for immediate loading onto an SDS-PAGE gel

TRUSTED_SUSTAINABILITY

Specifications

Tissue Embryonic Fibroblast
Preparation Method NIH 3T3 cells were Serum starved overnight and then incubated at room temperature for 10 min. in a final concentration of 1mM Sodium Vanadate, PDGF was then added at a final concentration of 5ng/mL and cells were incubated at 37°C
Quantity 500 μg
Storage Buffer 1mg/mL in SDS-PAGE Buffer: 62mM Tris pH 6.8, 2% SDS, 0.9% beta-mercaptoethanol, 0.003% Bromophenol Blue, 5% Glycerol
Host Species Mouse
Concentration 1 mg/mL
Content And Storage -20°C
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