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BD NIH 3T3 + Vanadate + PDGF Lysate

Useful control for the western blotting application

Manufacturer:  BD 612069

Catalog No. BDB612069


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Includes: Mouse Embryonic Fibroblast

Description

Description

  • Can be rich in endogenously expressed proteins
  • Convenient to use and provided in a denatured format allowing for immediate loading onto an SDS-PAGE gel

Specifications

Specifications

Embryonic Fibroblast
500μg
-20°C
1mg/mL
NIH 3T3 cells were Serum starved overnight and then incubated at room temperature for 10 min. in a final concentration of 1mM Sodium Vanadate, PDGF was then added at a final concentration of 5ng/mL and cells were incubated at 37°C
1mg/mL in SDS-PAGE Buffer: 62mM Tris pH 6.8, 2% SDS, 0.9% beta-mercaptoethanol, 0.003% Bromophenol Blue, 5% Glycerol
Mouse
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