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BD Sphero™ Rainbow Calibration Particles

Catalog No. BDB556298
Click to view available options
Diameter (Metric):
3 to 3.4 μm
6 to 6.4 μm
Description:
Blank Calibration Particles
Rainbow Calibration Particles
Rainbow Fluorescent Particles (High Intensity)
Rainbow Fluorescent Particles (Mid-range FL1 Fluorescence)

For flow cytometry calibration and alignment

  • For flow cytometry calibration and alignment
  • Have emission spectra compatible with many common fluorophores used for immunofluorescent staining with flow cytometric analysis
  • Very small coefficients of variation in both size and fluorescence
  • Contain water insoluble fluorescent dyes which are very stable, allowing them to be sterilized by washing with 70% alcohol
  • Provided in convenient dropper bottles containing 5mL of particle suspension at a concentration of 107 particles/mL
  • Polystyrene

Rainbow Calibration Particles (6 Peaks)

  • Each vial contains a mixture of Rainbow Particles that are dyed to six different fluorescent intensities
  • Rainbow Calibration Particles (8 Peaks)
    • The vial contains a mixture of Rainbow Particles that are dyed to eight different fluorescent intensities
  • Rainbow Fluorescent Particles (High Intensity)
    • Each vial contains a single population of Rainbow Particles that are dyed to a single fluorescent intensity
    • The intensity of this particle corresponds to the brightest intesity of peak in the Rainbow Calibration Particles, 3.0-3.4μm (Mfr. No. 556286)
    Rainbow Fluorescent Particles (Mid-range FL1 Fluorescence)
    • Each vial contains a single population of Rainbow Particles that are dyed to a single fluorescent intensity
  • Blank Calibration Particles
    • Each vial contains a single population of Blank Calibration Particles that are dyed to a single fluorescent intensity
    • The intensity of this particle corresponds to the dimmest intensity peak in the Rainbow Calibration Particles (6 Peaks), 6.0-6.4μm (Mfr. No. 5556288)
    • Approximate the background fluorescence of unstained cells

    TRUSTED_SUSTAINABILITY

    Specifications

    For Use With (Application) Flow Cytometry Calibration and Alignment
    Description Rainbow Fluorescent Particles (Mid-range FL1 Fluorescence)
    Concentration 107 particles/mL
    Certifications/Compliance 29 CFR 1910.1200, 91/155/EEC, 93/112/EC, RUO
    Diameter (Metric) 3 to 3.4 μm
    Content And Storage Aqueous solution containing 0.01%NP40 and ≤0.02% sodium azide
    Volume (Metric) 5 mL
    Color Clear
    For Use With (Equipment) Flow cytometer
    Includes Single population of rainbow particles that are dyed to a single fluorescent intensity
    Format Liquid
    Material Polystyrene
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