Flow Cytometry Compensation, Counting and Calibration
Invitrogen™ CountBright™ Plus Absolute Counting Beads
Calibrated suspension of ∼4 μm beads that encapsulate dyes that are excitable across a wide range of UV-IR wavelengths and brightly fluoresce in the emission range of 385-860 nm.
Invitrogen™ OneComp eBeads™ Compensation Beads
Spherical particles that can be stained with individual fluorochrome-conjugated antibodies for use as single-color compensation controls. OneComp eBeads react with antibodies of mouse, rat and hamster origin, and are immunoglobulin light chain-independent. 1 drop (50μL)/test
Invitrogen™ UltraComp eBeads™ Compensation Beads
Designed for use in compensation with all fluorochromes excited by ultraviolet, violet, blue, green, yellow-green, and red lasers. UltraComp eBeads™ react with antibodies of mouse, rat, and hamster origin, and are immunoglobulin light chain-independent. 1 drop (50μL)/test.
Invitrogen™ eBioscience™ Flow Cytometry Staining Buffer
A buffered saline solution containing fetal bovine serum and sodium azide (0.09%) as a preservative. Designed for use in immunofluorescent staining protocols of cells in suspension.
Molecular Probes™ CountBright™ Absolute Counting Beads, for flow cytometry
CountBright™ Absolute Counting Beads, for flow cytometry
Molecular Probes™ Flow Cytometry Sub-micron Particle Size Reference Kit
Flow Cytometry Sub-micron Particle Size Reference Kit
Invitrogen™ BrightComp eBeads™ Compensation Bead Kits
BrightComp eBeads compensation beads provide a consistent, accurate, and simple-to-use technique for setting flow cytometry compensation when using fluorescent protein-expressing samples.
Invitrogen™ 123count eBeads™ Counting Beads
Intended for use in absolute counting of cells or other particles by flow cytometry. 123count eBeads are 7μm microparticles containing encapsulated dyes compatible with blue (488nm) and violet (405nm) excitation sources and emitting fluorescence between approximately 500nm and 750nm.
Molecular Probes™ Alignflow™ Flow Cytometry Alignment Beads for Red Lasers, 6.0 μm
Alignflow™ Flow Cytometry Alignment Beads for Red Lasers, 6.0 μm
BD Sphero™ Fluorescent Particles
For flow cytometry calibration and alignment
Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set
Formulated and optimized for staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines
Invitrogen™ eBioscience™ Permeabilization Buffer (10X)
Can be used to fix and permeabilize cells prior to performing intracellular staining of cytokines and other cytoplasmic and/or nuclear antigens.
Invitrogen™ Cell Sorting Set-up Beads (for blue lasers)
Approximate the size, emission wavelength, and intensity of many biological samples, and so can be used to calibrate a flow cytometer’s cell sorting system, including laser source, optics, and stream flow.
Invitrogen™ CountBright™ Plus Ready Tubes
CountBright Plus Ready Tubes offer the same advantages as CountBright Plus Absolute Counting Beads, but in ready-to-use tubes. This not only improves the accuracy and repeatability of cell counting, but also allows for convenient room temperature storage for up to 12 months.
Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent
For staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines. Should be used with the Permeabilization Buffer. All three components sold together as the Foxp3 / Transcription Factor Staining Buffer Set.