Flow Cytometry Compensation, Counting and Calibration
Invitrogen™ OneComp eBeads™ Compensation Beads
Spherical particles that can be stained with individual fluorochrome-conjugated antibodies for use as single-color compensation controls. OneComp eBeads react with antibodies of mouse, rat and hamster origin, and are immunoglobulin light chain-independent. 1 drop (50μL)/test
Invitrogen™ BrightComp eBeads™ Compensation Bead Kits
BrightComp eBeads compensation beads provide a consistent, accurate, and simple-to-use technique for setting flow cytometry compensation when using fluorescent protein-expressing samples.
BD Sphero™ Fluorescent Particles
For flow cytometry calibration and alignment
Molecular Probes™ Cell Sorting Set-up Beads (for UV lasers)
Cell Sorting Set-up Beads (for UV lasers)
Invitrogen™ eBioscience™ Flow Cytometry Staining Buffer
A buffered saline solution containing fetal bovine serum and sodium azide (0.09%) as a preservative. Designed for use in immunofluorescent staining protocols of cells in suspension.
Invitrogen™ CountBright™ Plus Absolute Counting Beads
Calibrated suspension of ∼4 μm beads that encapsulate dyes that are excitable across a wide range of UV-IR wavelengths and brightly fluoresce in the emission range of 385-860 nm.
Molecular Probes™ Flow Cytometry Sub-micron Particle Size Reference Kit
Flow Cytometry Sub-micron Particle Size Reference Kit
Invitrogen™ eBioscience™ Human Regulatory T Cell Staining Kit #2
Contains all of the buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify regulatory T cells from human peripheral blood cells.
Invitrogen™ eBioscience™ Cell Stimulation Cocktail (plus protein transport inhibitors) (500X)
Can be used for induction and subsequent intracellular detection of cytokines and other secreted proteins in both in vitro-cultured and ex vivo cells
Invitrogen™ eBioscience™ Human Regulatory T Cell Whole Blood Staining Kit
Contains the buffers and monoclonal antibody necessary to successfully stain and identify Foxp3+ cells in whole blood samples.
Invitrogen™ eBioscience™ Intracellular Fixation & Permeabilization Buffer (plus Brefeldin A)
Designed for use in preparation of living cells for intracellular staining and flow cytometric analysis. A protein transport inhibitor (Brefeldin A) is included for treatment of cells during culture, to cause accumulation of cytokine protein at the Golgi and enhance resulting staining.
Invitrogen™ CountBright™ Plus Ready Tubes
CountBright Plus Ready Tubes offer the same advantages as CountBright Plus Absolute Counting Beads, but in ready-to-use tubes. This not only improves the accuracy and repeatability of cell counting, but also allows for convenient room temperature storage for up to 12 months.
Invitrogen™ eBioscience™ Cell Stimulation Cocktail (500X)
Cocktail of phorbol 12-myristate 13-acetate (PMA) and ionomycin. Treatment with PMA and ionomycin is sufficient to induce activation of many cell types to produce cytokines.
Invitrogen™ Cell Sorting Set-up Beads (for blue lasers)
Approximate the size, emission wavelength, and intensity of many biological samples, and so can be used to calibrate a flow cytometer’s cell sorting system, including laser source, optics, and stream flow.