Invitrogen™ BDNF Polyclonal Antibody

Catalog No.	Quantity
PIPA595183	100 μg

Catalog No. PIPA595183 Supplier Invitrogen™ Supplier No. PA595183

Description

Rabbit Polyclonal Antibody

Human BDNF shares 100% amino acid (aa) sequence identity with both mouse and rat BDNF. Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: recombinant human BDNF protein. IHC: mouse brain tissue, rat brain tissue . Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.

Specifications

• Antigen: BDNF
• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
• Classification: Polyclonal
• Concentration: 500 μg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 4mg trehalose and no preservative
• Gene: BDNF
• Gene Accession No.: P21237, P23363, P23560
• Gene Alias: abrineurin; ANON2; anorexia BDNF; Bdnf; BDNF precursor form; bdnf protein; brain derived neurothrophic factor; brain derived neurotrophic factor; brain-derived neurotrophic factor; BULN2; H-BDNF; neurotrophin; ProBDNF
• Gene Symbols: BDNF
• Host Species: Rabbit
• Immunogen: E. coli-derived human BDNF recombinant protein (Position: H129-R247).
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 12064, 24225, 627
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG

Safety and Handling

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