Invitrogen™ beta Amyloid (1-40) Polyclonal Antibody

Catalog No.	Quantity
44-136	100 μg

Catalog No. 44-136 Supplier Invitrogen™ Supplier No. 44136

Description

Rabbit Polyclonal Antibody

No significant cross-reactivity to beta-Amyloid 42 has been observed. Previous lots of this antibody recognized the sequence of beta-Amyloid [1-40] in the region from amino acids 15-30. No blocking activity was observed with beta-Amyloid [1-12] whereas the peptides beta-Amyloid [14-35], beta-Amyloid [15-28], beta-Amyloid [17-30] and Beta-Amyloid [1-40] were all able to block antibody activity.

Beta-amyloid is a 40-43 amino acid peptide cleaved from amyloid precursor protein, and a putative gamma-secretase. Amyloid beta (1-40) together with Amyloid beta (1-42) are two major C-terminal variants of the amyloid beta protein, constituting the majority of amyloid beta proteins. Both the amyloid beta (40) and amyloid beta (42) alloforms are major components of the extracellular plaques found in Alzheimer's disease brain tissue.

Specifications

• Antigen: beta Amyloid (1-40)
• Applications: Dot Blot, ELISA, Immunohistochemistry, Radioimmune Assays (RIA)
• Classification: Polyclonal
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with no preservative; pH 7.2
• Gene: APP
• Gene Accession No.: P05067
• Gene Alias: A beta 1 40; A β 1 40; A4; AAA; AB40; Abeta; Abeta40; Abeta42; ABPP; AD1; Adap; AG; AICD-50; AICD-57; AICD-59; AID(50); AID(57); AID(59); Alpha-CTF; Alpha-secretase C-terminal fragment; Alzheimer disease amyloid A4 protein homolog; alzheimer disease amyloid protein; Amyloid; Amyloid b; Amyloid beta; amyloid beta (A4) precursor protein; Amyloid beta 1 40; amyloid beta A4 protein; amyloid beta precursor protein; Amyloid intracellular domain 50; Amyloid intracellular domain 57; Amyloid intracellular domain 59; Amyloid precursor protein; Amyloid β; Amyloid-beta A4 protein; Amyloid-beta precursor protein; Amyloid-beta protein 40; Amyloid-beta protein 42; Amyloidogenic glycoprotein; App; APP-C57; APP-C59; APP-C99; APPI; appican; Aβ 1 40; B APP40; BAPP40; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); beta-amyloid precursor protein; betaApp; BetaAPP40; Beta-APP40; Beta-APP42; Beta-CTF; Beta-secretase C-terminal fragment; C31; C80; C83; C99; Cerebral vascular amyloid peptide; CTFgamma; CVAP; E030013M08Rik; Gamma-CTF(50); Gamma-CTF(57); Gamma-CTF(59); Gamma-secretase C-terminal fragment 50; Gamma-secretase C-terminal fragment 57; Gamma-secretase C-terminal fragment 59; N-APP; P3(40); P3(42); peptidase nexin-II; PN2; PN-II; PreA4; protease nexin II; protease nexin-II; S-APP-alpha; S-APP-beta; Soluble APP-alpha; Soluble APP-beta; β APP40; βAPP40; testicular tissue protein Li 2
• Gene Symbols: APP
• Host Species: Rabbit
• Immunogen: The antiserum was produced against a chemically synthesized peptide corresponding to amino acids 1-40 of human beta-Amyloid (A-beta).
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 351
• Target Species: Human
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG

Frequently Asked Questions (FAQs)

I know that there's no single procedure that applies to all antigens, but what are some commonly used procedures for performing immunohistochemical staining of brain tissue?

Here is a procedure for the immunohistochemical staining of Beta-amyloid with paraffin embedded sections of transgenic mouse brain. The following protocol was developed for use with paraffin embedded sections stained with a variety of our antibodies against Beta-amyloid. Applicable rabbit and mouse anti-Beta-amyloid antibodies are: 51-2700, 13-0200, 71-5800, 13-0100Z, 37-4200, 43-7900, 44-136, 700254, 36-6900, and AHB0121.

Beta-amyloid Staining:
- Transgenic mice (expressing the transgenes PS1-A246E + APP swe, APP swe alone, or PS1-A246E alone) were perfused with 1 x Dulbecco's phosphate buffered saline (D-PBS) followed by 4% paraformldehyde buffered with D-PBS. Brain tissues were then embedded in paraffin prior to microtome sectioning.
- After mounting on slides, the paraffin-embedded tissue sections were then deparaffinized with heat. The sections were then incubated for 3 minutes in 70% formic acid. Next, they were deparaffinized further with xylene followed with 100% ethanol. The sections were then re-hydrated in a graded ethanol series (100% ethanol, 95% ethanol, 70% ethanol, and then water).
- Endogenous peroxidase activity was quenched with 3% hydrogen peroxide in methanol. The sections were then heated in the microwave for 5-7 minutes in water, cooled at room temperature for 5 minutes and then rinsed in water. The sections were then washed in TBS (0.05 M Tris-HCl, pH 7.6, with 0.25 M NaCl) prior to blocking. Non-specific binding was blocked with 3% normal goat serum and 0.1% Triton X-100 in TBS for 1 hour at room temperature.
- The sections were then stained with anti-Beta-amyloid antibodies. The staining solution typically consisted of the antibody at a concentration of 5 µg/mL in TBS containing 2% normal goat serum. After incubation at room temperature for at least 1 hr., the sections were washed in TBS 3 times for 5 minutes each. An anti-mouse or anti-rabbit secondary antibody labeled with HRP was then used with DAB or AEC as substrates to stain the Beta-amyloid.
[Adapted from Borchelt, D.R., et al. (1997) Accelerated amyloid deposition in the brains of transgenic mice coexpressing mutant presenilin 1 and amyloid precursor proteins. Neuron 19:939-945.]

Tau and Synuclein Staining:
Here is a general procedure for the immunohistochemical staining of tau, its phosphorylated forms, and synuclein in frozen sections of rat brain. This protocol is adapted from one kindly contributed by Dr. Emil Adamec, M.D., Ph.D., McLean Hospital, Belmont, MA. The procedure was developed for use with cryostat sections of rat brain.
- Sections were fixed with 4% paraformaldehyde for 25 minutes. Sections were then extracted with 0.01% (v/v) Triton X-100. Endogenous peroxidase was blocked with 3% hydrogen peroxide. Sections were then treated with 80% formic acid for 5 to 10 minutes at room temperature to enhance staining. Note that formic acid treatment is also very useful prior to staining with anti-synuclein antibodies.
- The various antibodies were used at dilutions of 1:100 to 1:250. The sections were incubated with the diluted primary antibody overnight at 4°C. A species-specific secondary antibody labeled with HRP was then used with either DAB or AEC to stain the antigens.
- Applicable rabbit and mouse anti-tau and phospho-tau antibodies are: AHB0042, AHB0061, 44-738G, 39-1800, 13-6400, 13-1400, and 18-7461. Anti-synuclein antibodies useful for IHC are: 18-0215, 18-7461, 32-8100, 32-8200, 32-8500, 35-8300, 35-8400, 39-1800, and AHB0261.