beta-Phosphoglucomutase Polyclonal Antibody, Biotin, Rockland™

Goat Polyclonal Antibody

Supplier:  Rockland Immunochemicals 2001062370100

Catalog No. 50-199-0427

Description

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Anti-Beta-Phosphoglucomutase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Beta-Phosphoglucomutase [Lactococcus lacti]. Cross reactivity against Beta-Phosphoglucomutase from other sources is unknown.

Beta-Phosphoglucomutase catalyzes the interconversion of D-glucose 1-phosphate (G1P) and D-glucose 6-phosphate (G6P), forming beta-D-glucose 1,6-(bis)phosphate (beta-G16P) as an intermediate. The beta-phosphoglucomutase (Beta-PGM) acts on the beta-C1 anomer of G1P. Glucose or lactose are used in preference to maltose, which is only utilized after glucose or lactose has been exhausted. It plays a key role in the regulation of the flow of carbohydrate intermediates in glycolysis and the formation of the sugar nucleotide UDP-glucose. The catalysis proceeds via a phosphoenzyme formed by reaction of an active-site nucleophile with the cofactor glucose 1,6-diphosphate (G1,6-diP). The phosphorylated mutase binds either G1P or G6P and transfers the phosphoryl group to the C6OH or C1OH, respectively.

Specifications

• Antigen: beta-Phosphoglucomutase
• Classification: Polyclonal
• Conjugate: Biotin
• Host Species: Goat
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Lyophilized

• Applications: ELISA, Immunoprecipitation, Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
• Immunogen: beta Phosphoglucomutase [Lactococcus lacti]
• Regulatory Status: RUO
• Target Species: Bacteria
• Product Type: Antibody